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PMID: 3387185 Published · ppublish English Journal Article

Sodium and calcium currents of acutely isolated adult rat superior cervical ganglion neurons.

Pflugers Archiv : European journal of physiology ·Vol. 411 ·No. 5 ·1988-05-00 ·Pages 481-90

Schofield GG, Ikeda SR

Abstract

Neurons enzymatically isolated from the adult rat superior cervical ganglion (SCG) were investigated using the whole-cell variant of the patch-clamp technique. Current-clamp studies revealed the following mean passive and active membrane properties: resting membrane potential, -54.9 mV; input resistance, 349 M omega; action potential (AP) threshold, -29.8 mV; AP overshoot, 53.3 mV; AP maximum rate of rise, 166.4 V/s; and AP duration, 3.2 ms. Chemosensitivity to acetylcholine remained intact following enzymatic dispersion. Voltage-clamp studies of a transient tetrodotoxin-sensitive Na+ current revealed activation and inactivation processes which could be fit to modified Boltzmann equations. Na+ current activation parameters for the half activation potential (Vh) and slope factor (K) were -23.3 mV and 5.3 mV, respectively. Inactivation parameters for Vh and K were -59.3 mV and 7.6 mV, respectively. Voltage-clamp studies also revealed a high voltage-activated sustained inward current which was eliminated upon removal of external Ca2+, greatly reduced by 500 microM Cd2+, and supported by Ba2+ or Sr2+. Tail current analysis of this Ca2+ current revealed a sigmoidal activation. A low voltage-activated transient Ca2+ current was not observed. We conclude that isolated SCG neurons retain the properties of neurons in intact ganglia and provide several advantages over conventional preparations for the study of voltage-gated membrane currents.

MeSH Terms
Action Potentials Animals Calcium/physiology Ganglia, Sympathetic/physiology In Vitro Techniques Male Membrane Potentials Microelectrodes Neurons/physiology Rats Rats, Inbred Strains Sodium/physiology
Chemicals
Sodium Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schofield G G
Section of Electrophysiology, National Institute on Alcohol Abuse and Alcoholism, Rockville, MD 20852.
Ikeda S R
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00582368
Published
1988-05-00
Pages
481-90
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00582368 View journal details
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