Home LiteratureArticle Details
PMID: 3380801 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Reversal of the metastatic phenotype in Lewis lung carcinoma cells after transfection with syngeneic H-2Kb gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 12 ·1988-06-00 ·Pages 4463-7

Plaksin D, Gelber C, Feldman M, Eisenbach L

Abstract

High metastatic clones of the murine 3LL carcinoma express greatly reduced levels of H-2Kb major histocompatibility complex class I antigens, while low metastatic clones of the same tumor express high levels of H-2Kb. Induced expression of this antigen after transfection with the H-2Kb gene resulted in conversion of a metastatic to a non- or low-metastatic phenotype. Unlike the parental cells, transfected cells are potent inducers of H-2Kb-restricted syngeneic cytotoxic lymphocytes that kill the Kb-positive clones and cross-react with parental nontransfected cells. Preimmunization of mice with Kb-positive transfectants conferred protection against metastatic spread of malignant cells. Moreover, immunotherapy of metastasis was achieved by immunization with the H-2Kb-transfected cells of animals already carrying a growing local tumor of the parental cells.

MeSH Terms
Animals Genes, MHC Class I H-2 Antigens/genetics Lung Neoplasms/immunology,pathology Mice Mice, Inbred C3H Mice, Inbred C57BL Neoplasm Metastasis Neoplasm Transplantation Nucleic Acid Hybridization Phenotype Plasmids Transcription, Genetic Transfection Transplantation, Isogeneic
Chemicals
H-2 Antigens H-2Kb protein, mouse
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Plaksin D
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Gelber C
Feldman M
Eisenbach L
References (29)
29 references, click to expand
  1. The DNA sequence of the H-2kb gene: evidence for gene conversion as a mechanism for the generation of polymorphism in histocompatibilty antigens.
    EMBO J. 1983;2(3):453-62 PMID: 11894963
  2. Down-regulation of class I HLA antigens and of the Epstein-Barr virus-encoded latent membrane protein in Burkitt lymphoma lines.
    Proc Natl Acad Sci U S A. 1987 Jul;84(13):4567-71 PMID: 3037521
  3. Mouse teratocarcinoma and embryonic antigens.
    Immunol Rev. 1977 Jan;33:3-32 PMID: 66184
  4. Major histocompatibility complex restricted cell-mediated immunity.
    Adv Immunol. 1977;25:55-91 PMID: 76435
  5. MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness.
    Adv Immunol. 1979;27:51-177 PMID: 92183
  6. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  7. Differential expression of H-2 gene products in tumour cells in associated with their metastatogenic properties.
    Nature. 1980 Nov 13;288(5787):179-81 PMID: 7432518
  8. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression.
    J Immunol. 1981 Jan;126(1):317-21 PMID: 6935293
  9. Control of progression of local tumor and pulmonary metastasis of the 3LL Lewis lung carcinoma by different histocompatibility requirements in mice.
    J Natl Cancer Inst. 1981 May;66(5):919-26 PMID: 6939934
  10. Variation in MHC antigenic profiles of tumor cells and its biological effects.
    Immunol Rev. 1981;60:85-127 PMID: 6171505
  11. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  12. Resistance to cell-mediated cytotoxicity is correlated with reduction of H-2K gene products in AKR leukemia.
    Immunogenetics. 1982;16(3):257-64 PMID: 6982849
  13. A cDNA clone containing the entire coding sequence of a mouse H-2Kd histocompatibility antigen.
    Nucleic Acids Res. 1983 Mar 11;11(5):1567-77 PMID: 6298749
  14. Striking paucity of HLA-A, B, C and beta 2-microglobulin on human neuroblastoma cell lines.
    J Immunol. 1983 May;130(5):2471-8 PMID: 6187860
  15. MHC imbalance and metastatic spread in Lewis lung carcinoma clones.
    Int J Cancer. 1983 Jul 15;32(1):113-20 PMID: 6862690
  16. Loss of expression of transplantation antigens encoded by the H-2K locus on Lewis lung carcinoma cells and its relevance to the tumor's metastatic properties.
    J Natl Cancer Inst. 1983 Jul;71(1):139-45 PMID: 6345878
  17. Expression of class I major histocompatibility antigens switched off by highly oncogenic adenovirus 12 in transformed rat cells.
    Nature. 1983 Oct 27-Nov 2;305(5937):771-5 PMID: 6355856
  18. Tumorigenicity of cells transformed by adenovirus type 12 by evasion of T-cell immunity.
    Nature. 1983 Oct 27-Nov 2;305(5937):776-9 PMID: 6605479
  19. Absence of human leukocyte antigen molecules in skin tumors and some cutaneous appendages: evidence using monoclonal antibodies.
    J Am Acad Dermatol. 1983 Dec;9(6):867-71 PMID: 6196384
  20. Alternative RNA splicing in expression of the H-2K gene.
    Nature. 1983 Dec 8-14;306(5943):602-4 PMID: 6689056
  21. Immuno-selection in vivo of H-2D phenotypic variants from a metastatic clone of sarcoma cells results in cell lines of altered metastatic competence.
    Int J Cancer. 1984 Mar 15;33(3):407-15 PMID: 6698640
  22. Target sites for antibodies and cytotoxic T lymphocytes on H-2 molecules.
    Transplant Proc. 1983 Dec;15(4):2093-6 PMID: 6200977
  23. The differential expression of H-2K versus H-2D antigens, distinguishing high-metastatic from low-metastatic clones, is correlated with the immunogenic properties of the tumor cells.
    Int J Cancer. 1984 Oct 15;34(4):567-73 PMID: 6490207
  24. Rejection of transplantable AKR leukaemia cells following MHC DNA-mediated cell transformation.
    Nature. 1984 Oct 25-31;311(5988):750-2 PMID: 6333639
  25. Markedly decreased expression of class I histocompatibility antigens, protein, and mRNA in human small-cell lung cancer.
    J Exp Med. 1985 May 1;161(5):1135-51 PMID: 2580935
  26. Abrogation of metastatic properties of tumour cells by de novo expression of H-2K antigens following H-2 gene transfection.
    Nature. 1985 May 23-29;315(6017):301-5 PMID: 3873616
  27. Alternative protein products with different carboxyl termini from a single class I gene, H-2Kb.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):6084-8 PMID: 3461476
  28. Expression of major histocompatibility complex class I antigens as a strategy for the potentiation of immune recognition of tumor cells.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8723-7 PMID: 3464979
  29. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.12.4463
Published
1988-06-00
Pages
4463-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280450
Subset
IM
Grants
NCI NIH HHS · CA 28139 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.12.4463 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2