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PMID: 2580935 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Markedly decreased expression of class I histocompatibility antigens, protein, and mRNA in human small-cell lung cancer.

The Journal of experimental medicine ·Vol. 161 ·No. 5 ·1985-05-01 ·Pages 1135-51

Doyle A, Martin WJ, Funa K, Gazdar A, Carney D, Martin SE, Linnoila I, Cuttitta F, Mulshine J, Bunn P

Abstract

We have found markedly deficient expression of the class I major histocompatibility antigens HLA-A,B,C and beta 2m on human small-cell lung cancer (SCLC) lines and fresh tumor samples. The deficit of HLA-A,B,C and beta 2-microglobulin (beta 2m) antigen expression was demonstrated with both radiobinding assays and indirect immunofluorescence assays. Immunoprecipitation of metabolically labeled cells with antibodies to class I antigens showed most SCLC lines to have synthesized almost no beta 2m and HLA-A,B,C proteins. Northern blot analysis, using human HLA-A,B, and beta 2m cDNA probes, showed that almost all SCLC lines tested had markedly decreased amounts of HLA and beta 2m mRNA, but both gene products could be induced with interferon treatment of SCLC lines. We conclude that human SCLC, in contrast to other lung cancer types, is characterized by greatly reduced transcription of HLA-A,B,C and beta 2m genes, which suggests the existence of a mechanism for evading the host immune response to the tumor and of an E1a-like product in this type of tumor cell.

MeSH Terms
Antigens, Surface/analysis Carcinoma, Small Cell/genetics,immunology,metabolism Cell Line Genes, MHC Class II HLA Antigens/analysis,biosynthesis,genetics HLA-A Antigens HLA-B Antigens HLA-C Antigens Humans Interferons/pharmacology Lung Neoplasms/genetics,immunology,metabolism RNA, Messenger/biosynthesis beta 2-Microglobulin/biosynthesis,genetics,immunology
Chemicals
Antigens, Surface HLA Antigens HLA-A Antigens HLA-B Antigens HLA-C Antigens RNA, Messenger beta 2-Microglobulin Interferons
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Doyle A
Martin W J
Funa K
Gazdar A
Carney D
Martin S E
Linnoila I
Cuttitta F
Mulshine J
Bunn P
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.161.5.1135
Published
1985-05-01
Pages
1135-51
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187608
Subset
IM
Grants
NCI NIH HHS · CA 33693 · United States
External Links
PubMed source DOI: 10.1084/jem.161.5.1135 PMC Full Text View journal details
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