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PMID: 337301 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Escherichia coli ribosomal protein S1 has two polynucleotide binding sites.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 11 ·1977-11-00 ·Pages 4786-90

Draper DE, Pratt CW, von Hippel PH

Abstract

The interaction of Escherichia coli ribosomal protein S1 with a variety of RNA and DNA oligomers and polymers has been studied, using both a sedimentation technique and the quenching of intrinsic protein fluorescence upon nucleic acid binding to obtain equilibrium binding parameters. Two polynucleotide binding sites have been detected on S1: site I binds either single-stranded DNA or RNA and does not discriminate between adenine- and cytidine-containing polynucleotides, while the II binding is highly specific for RNA over DNA and shows a marked preference for cytidine polynucleotides over the corresponding adenine-containing species. On the basis of the binding properties of S1 to denatured DNA cellulose and poly(rC)-cellulose, it is demonstrated that every S1 molecule carries both a site I and a site II. Some possible implications of these results for mechanisms of protein synthesis and phage Qbeta replication are briefly considered.

MeSH Terms
Bacterial Proteins/metabolism Binding Sites Centrifugation, Density Gradient DNA, Bacterial/metabolism Escherichia coli/metabolism,ultrastructure Polynucleotides/metabolism RNA, Bacterial/metabolism Ribosomal Proteins/metabolism Spectrometry, Fluorescence
Chemicals
Bacterial Proteins DNA, Bacterial Polynucleotides RNA, Bacterial Ribosomal Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Draper D E
Pratt C W
von Hippel P H
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.11.4786
Published
1977-11-00
Pages
4786-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432040
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.11.4786 PMC Full Text View journal details
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