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PMID: 337127 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Multiple effects of an RNA polymerase beta' mutation on in vitro transcription.

Molecular & general genetics : MGG ·Vol. 155 ·No. 2 ·1977-10-20 ·Pages 191-6

Young BS, Wright A

Abstract

S. tyrphimurium strain BY324 is temperature sensitive due to a mutation (rpo C32) in the gene for the RNA polymerase beta' subunit. Transcription of T7 DNA by RNA polymerase purified from this strain is temperature sensitive in vitro. The enzyme is slightly defective in template binding and RNA chain initiation, but the major defect is in RNA chain elongation. The rate of RNA chain elongation is reduced 4-5 fold relative to wild-type. RNA chain termination does not appear to be affected by the beta' mutation. While the elongation defect is suppressed by glycerol or dimethylsulfoxide, the initiation defect is not. Possible roles for the beta' subunit in enzyme function are discussed in light of these results.

MeSH Terms
DNA-Directed RNA Polymerases/genetics Enzyme Precursors/genetics Mutation RNA, Bacterial/genetics Salmonella typhimurium/enzymology,genetics Transcription, Genetic
Chemicals
Enzyme Precursors RNA, Bacterial DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Young B S
Wright A
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22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00393159
Published
1977-10-20
Pages
191-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00393159
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