A temperature sensitive mutation of the beta'-subunit of DNA-dependent RNA polymerase from E. coli T 16.

Ammonium Chloride/pharmacology Cellulose Chromatography, Affinity Chromatography, Gel Coliphages DNA Viruses DNA, Viral DNA-Directed RNA Polymerases/isolation & purification,metabolism Drug Stability Electrophoresis Electrophoresis, Polyacrylamide Gel Enzyme Activation/drug effects Escherichia coli/drug effects,enzymology Hydrogen-Ion Concentration Kinetics Macromolecular Substances Mutation Protein Binding Temperature Time Factors

DNA, Viral Macromolecular Substances Ammonium Chloride Cellulose DNA-Directed RNA Polymerases