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PMID: 3357893 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Neural regulation of acetylcholine sensitivity in embryonic sympathetic neurons.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 8 ·1988-04-00 ·Pages 2825-9

Role LW

Abstract

The development of transmitter sensitivity is an important component of synaptic differentiation. Despite a wealth of information about the appearance of acetylcholine (AcCho) sensitivity at the neuromuscular junction, the onset and regulation of this critical aspect of synaptogenesis has not previously been examined for synapse formation between neurons. To determine whether there is a role of presynaptic input in the induction of AcCho sensitivity at interneuronal synapses, AcCho-induced currents were measured in embryonic sympathetic neurons before and after synapse formation in vitro. The total AcCho sensitivity of postsynaptic neurons was increased nearly 10-fold after innervation. The effects of innervation are mimicked by medium conditioned by preganglionic neurons, suggesting that presynaptic neurons regulate postsynaptic AcCho sensitivity by release of a soluble factor. These observations provide evidence that presynaptic input regulates neuronal sensitivity to an identified synaptic transmitter.

MeSH Terms
Acetylcholine/pharmacology Animals Chick Embryo Dose-Response Relationship, Drug Electric Conductivity In Vitro Techniques Membrane Potentials/drug effects Neural Pathways/embryology Spinal Cord/embryology Sympathetic Nervous System/embryology,physiology Synapses/physiology
Chemicals
Acetylcholine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Role L W
Columbia University, College of Physicians and Surgeons, Department of Anatomy and Cell Biology, New York, NY 10032.
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.8.2825
Published
1988-04-00
Pages
2825-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280092
Subset
IM
Grants
NINDS NIH HHS · NS22061 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.8.2825 PMC Full Text View journal details
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