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PMID: 3356467 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A galE via (Vi antigen-negative) mutant of Salmonella typhi Ty2 retains virulence in humans.

Infection and immunity ·Vol. 56 ·No. 5 ·1988-05-00 ·Pages 1326-33

Hone DM, Attridge SR, Forrest B, Morona R, Daniels D, LaBrooy JT, Bartholomeusz RC, Shearman DJ, Hackett J

Abstract

We have recently described the construction of a galE derivative of Salmonella typhi Ty2 (Ty2H1) which had a 0.4-kilobase deletion in the galE gene and was sensitive to galactose-induced lysis when cultured with greater than or equal to 0.06 mM galactose (D. M. Hone, R. Morona, S. Attridge, and J. Hackett, J. Infect. Dis. 156:167-174, 1987). We now report the selection of a rifampin-resistant, via derivative of Ty2H1, EX462. Compared with the Ty2 parent strain, EX462 was serum sensitive and highly attenuated in the mouse mucin virulence assay. When four human volunteers ingested 7 X 10(8) viable EX462, two became ill and developed a typhoidlike disease with fever and bacteremia. Blood isolates from these individuals were indistinguishable from the vaccine strain by a variety of criteria. We concluded that, even in a via background, the galE mutation was not attenuating for S. typhi in humans.

MeSH Terms
Animals Antibodies, Bacterial/biosynthesis Antigens, Bacterial/genetics,immunology Bacterial Vaccines/immunology Blood Bactericidal Activity Disease Models, Animal Galactose/metabolism Genes, Bacterial Humans In Vitro Techniques Lipopolysaccharides/metabolism Mice Mutation Salmonella typhi/genetics,pathogenicity Species Specificity
Chemicals
Antibodies, Bacterial Antigens, Bacterial Bacterial Vaccines Lipopolysaccharides Galactose
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Hone D M
Department of Microbiology and Immunology, University of Adelaide, South Australia.
Attridge S R
Forrest B
Morona R
Daniels D
LaBrooy J T
Bartholomeusz R C
Shearman D J
Hackett J
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.5.1326-1333.1988
Published
1988-05-00
Pages
1326-33
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259821
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.56.5.1326-1333.1988 PMC Full Text View journal details
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