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PMID: 3329053 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nuclearly-encoded CBP1 interacts with the 5' end of mitochondrial cytochrome b pre-mRNA.

Current genetics ·Vol. 12 ·No. 6 ·1987-00-00 ·Pages 391-7

Dieckmann CL, Mittelmeier TM

Abstract

CBP1 is a nuclearly-encoded protein that is imported into mitochondria and confers stability on the mRNA for cytochrome b. Previous work has shown that CBP1 interacts with the cytochrome b transcript upstream of the coding sequence; a region encompassing some 1,100 nucleotides. The work presented here narrows the site of action of CBP1 to the distal third of this upstream sequence through analysis of mRNA produced from a novel recombinant gene containing segments of the gene for cytochrome b, cob, and the ATP synthase subunit 9 gene, olil. In a wild-type CBP1 strain, the cob-olil-cob gene produces stable, mature mRNA that is translated and contributes a portion of the cytochrome b necessary for optimal growth on non-fermentable medium.

MeSH Terms
Cell Nucleus/metabolism Cytochrome b Group/genetics Fungal Proteins/genetics Genes Genes, Fungal Genotype Mitochondria/metabolism RNA Precursors/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/genetics,metabolism
Chemicals
Cytochrome b Group Fungal Proteins RNA Precursors RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dieckmann C L
Department of Biochemistry, University of Arizona, Tucson 85721.
Mittelmeier T M
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16 references, click to expand
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00434815
Published
1987-00-00
Pages
391-7
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Grants
NIGMS NIH HHS · GM34893 · United States
External Links
PubMed source DOI: 10.1007/BF00434815 View journal details
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