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PMID: 3329049 Published · ppublish English Journal Article

DNA repair genes of Saccharomyces cerevisiae: complementing rad4 and rev2 mutations by plasmids which cannot be propagated in Escherichia coli.

Current genetics ·Vol. 11 ·No. 3 ·1986-00-00 ·Pages 205-10

Siede W, Eckardt-Schupp F

Abstract

The RAD4 gene of yeast required for the incision step of DNA excision repair and the REV2 (= RAD5) gene involved in mutagenic DNA repair could not be isolated from genomic libraries propagated in E. coli regardless of copy number of the shuttle vector in yeast. Transformants with plasmids conferring UV resistance to a rad4-4 or a rev2-1 mutant were only recovered if yeast was transformed directly without previous amplification of the gene bank in E. coli. DNA preparations from these yeast clones yielded no transformants in E. coli but retransformation of yeast was possible. This lead to the isolation of a defective derivative of the rad4 complementing plasmid. The modified plasmid was now capable of transforming E. coli but still interfered significantly with its growth.

MeSH Terms
Cloning, Molecular DNA Repair Escherichia coli/genetics Genes, Fungal Mutation Plasmids Saccharomyces cerevisiae/genetics,radiation effects Ultraviolet Rays
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Siede W
Gesellschaft für Strahlen- und Umweltforschung, Institut für Strahlenbiologie, Neuherberg, Federal Republic of Germany.
Eckardt-Schupp F
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00420608
Published
1986-00-00
Pages
205-10
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00420608 View journal details
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