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PMID: 3329040 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Alkylation mutagenesis in Saccharomyces cerevisiae: lack of evidence for an adaptive response.

Current genetics ·Vol. 10 ·No. 9 ·1986-00-00 ·Pages 647-55

Polakowska R, Perozzi G, Prakash L

Abstract

We have found no evidence for an adaptive response for either lethality or mutagenesis following treatment of Saccharomyces cerevisiae with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The rad6 and rad52 mutants of S. cerevisiae are highly defective in MNNG and ethyl methanesulfonate induced mutagenesis of both stationary and exponential phase cells. These and other observations indicate that the mechanisms of repair of alkylation damage and mutagenesis differ markedly between S. cerevisiae and Escherichia coli.

MeSH Terms

Adaptation, Physiological Alkylation Cell Cycle Cycloheximide/pharmacology DNA Repair/drug effects Ethyl Methanesulfonate Genes, Fungal Methylnitronitrosoguanidine Mutation Saccharomyces cerevisiae/cytology,drug effects,genetics

Chemicals

Methylnitronitrosoguanidine Cycloheximide Ethyl Methanesulfonate

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Polakowska R

Department of Radiation Biology and Biophysics, University of Rochester School of Medicine, NY 14642.

Perozzi G

Prakash L

References (28)

28 references, click to expand

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Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00410912

Published

1986-00-00

Pages

647-55

Language

English

Region

United States

NLM ID

8004904

Subset

Grants

NIEHS NIH HHS · ES 02433 · United States

External Links

PubMed source DOI: 10.1007/BF00410912 View journal details

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Alkylation mutagenesis in Saccharomyces cerevisiae: lack of evidence for an adaptive response.

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