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PMID: 3320059 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Translocation in yeast and mammalian cells: not all signal sequences are functionally equivalent.

The Journal of cell biology ·Vol. 105 ·No. 6 Pt 2 ·1987-12-00 ·Pages 2905-14

Bird P, Gething MJ, Sambrook J

Abstract

In Saccharomyces cerevisiae, nascent carboxypeptidase Y (CPY) is directed into the endoplasmic reticulum by an NH2-terminal signal peptide that is removed before the glycosylated protein is transported to the vacuole. In this paper, we show that this signal peptide does not function in mammalian cells: CPY expressed in COS-1 cells is not glycosylated, does not associate with membranes, and retains its signal peptide. In a mammalian cell-free protein-synthesizing system, CPY is not translocated into microsomes. However, if the CPY signal is either mutated to increase its hydrophobicity or replaced with that of influenza virus hemagglutinin, the resulting precursors are efficiently translocated both in vivo and in vitro. The implications of these results for models of signal sequence function are discussed.

MeSH Terms
Animals Biological Transport Carboxypeptidases/metabolism Cathepsin A Cell Line Chlorocebus aethiops DNA Mutational Analysis Endoplasmic Reticulum/metabolism Glycosylation Hemagglutinins, Viral/metabolism Microsomes/metabolism Protein Processing, Post-Translational Protein Sorting Signals/physiology Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Solubility Structure-Activity Relationship
Chemicals
Hemagglutinins, Viral Protein Sorting Signals Saccharomyces cerevisiae Proteins Carboxypeptidases Cathepsin A PRC1 protein, S cerevisiae serine carboxypeptidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bird P
Department of Biochemistry, University of Texas Health Center, Dallas 75235.
Gething M J
Sambrook J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.105.6.2905
Published
1987-12-00
Pages
2905-14
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114712
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.105.6.2905 PMC Full Text View journal details
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