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PMID: 3318809 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A re-assessment of the role of protein kinase C in glucose-stimulated insulin secretion.

The Biochemical journal ·Vol. 246 ·No. 2 ·1987-09-01 ·Pages 489-93

Hii CS, Jones PM, Persaud SJ, Howell SL

Abstract

Isolated rat islets of Langerhans which had been pretreated with 200 nM-phorbol 12-myristate 13-acetate (PMA) for 20-24 h, a treatment reported in other cell types to deplete cells of protein kinase C activity, were found not to contain detectable Ca2+/phospholipid-dependent protein kinase activity. These islets did not secrete insulin in response to a subsequent exposure to PMA (0.1 or 1 microM) during a 30 min incubation, although insulin secretion could be stimulated by 20 mM-glucose, a response which was enhanced by 20 microM-forskolin. PMA-pretreated islets that had been permeabilized by high-voltage discharge showed unimpaired secretory responses to an increase in Ca2+ concentration, cyclic AMP and forskolin. These results suggest that (i) pretreatment of islets with tumour-promoting phorbol esters may be a useful means of investigating the role of protein kinase C in stimulus-secretion coupling in the pancreatic beta-cell and (ii) protein kinase C may not play an essential role in glucose-induced insulin secretion.

MeSH Terms
Animals Colforsin/pharmacology In Vitro Techniques Insulin/metabolism Insulin Secretion Islets of Langerhans/drug effects,enzymology,metabolism Phosphorylation Protein Kinase C/metabolism Proteins/metabolism Rats Rats, Inbred Strains Sucrose/pharmacology Tetradecanoylphorbol Acetate/pharmacology
Chemicals
Insulin Proteins Colforsin Sucrose Protein Kinase C Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hii C S
Department of Physiology, Kings College London, University of London, U.K.
Jones P M
Persaud S J
Howell S L
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32 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2460489
Published
1987-09-01
Pages
489-93
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148300
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2460489 PMC Full Text View journal details
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