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PMID: 3302596 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA sequence analysis of spontaneous mutation in a PolA1 strain of Escherichia coli indicates sequence-specific effects.

Molecular & general genetics : MGG ·Vol. 207 ·No. 2-3 ·1987-05-00 ·Pages 267-72

Fix DF, Burns PA, Glickman BW

Abstract

The sequences of a collection of 261 spontaneous lacI- mutants recovered in a PolA- strain of Escherichia coli have indicated an increase in the frequency of most classes of mutation in this strain. Among base substitutions in lacI, a preference for transversions over transitions was observed. In addition, a single transition in the lac operator was enhanced 8-fold. More significantly, of 18 frameshifts, 12 occurred adjacent to a 5'-GTGG-3' sequence. Likewise, 15 of 24 deletions and 2 of 10 duplications had 5'-GTGG-3' sequences at one or both endpoints. We speculate that the prevalence of mutations at these specific sequences reflects the persistence of strand discontinuities that enhance the opportunity for mutagenic mishaps. Further, 5'-GTGG-3' sequences apparently represent sites where DNA polymerase I is involved in some aspect of DNA metabolism. These results strengthen the view that DNA context contributes an important component to spontaneous mutagenesis and indicate an anti-mutagenic role for DNA polymerase I.

MeSH Terms
Base Sequence DNA, Bacterial/genetics Escherichia coli/genetics Mutation Species Specificity
Chemicals
DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fix D F
Burns P A
Glickman B W
References (36)
36 references, click to expand
  1. Genetic studies of the lac repressor. VII. On the molecular nature of spontaneous hotspots in the lacI gene of Escherichia coli.
    J Mol Biol. 1978 Dec 25;126(4):847-57 PMID: 370408
  2. Complementary base pairing and the origin of substitution mutations.
    Nature. 1976 Sep 23;263(5575):285-9 PMID: 958482
  3. Model for the participation of quasi-palindromic DNA sequences in frameshift mutation.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4128-32 PMID: 7051004
  4. Increased frequency of deletions in DNA polymerase mutants of Escherichia coli.
    Nature. 1970 Nov 14;228(5272):633-5 PMID: 4920019
  5. Mechanism of mutation by thymine starvation in Escherichia coli: clues from mutagenic specificity.
    J Bacteriol. 1985 Jun;162(3):859-64 PMID: 3888966
  6. Genetic studies of the lac repressor. II. Fine structure deletion map of the lacI gene, and its correlation with the physical map.
    J Mol Biol. 1977 Jan 15;109(2):303-26 PMID: 839544
  7. Much of spontaneous mutagenesis in Escherichia coli is due to error-prone DNA repair: implications for spontaneous carcinogenesis.
    Carcinogenesis. 1981;2(9):863-72 PMID: 7028309
  8. Acetylornithinase of Escherichia coli: partial purification and some properties.
    J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
  9. Powerful mutator activity of the polA1 mutation within the histidine region of Escherichia coli K-12.
    J Bacteriol. 1982 Mar;149(3):955-60 PMID: 7037745
  10. Correlation of lac operator DNA imino proton exchange kinetics with its function.
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3665-9 PMID: 6328523
  11. Molecular basis of base substitution hotspots in Escherichia coli.
    Nature. 1978 Aug 24;274(5673):775-80 PMID: 355893
  12. Genetic mapping and DNA sequence analysis of mutations in the polA gene of Escherichia coli.
    J Mol Biol. 1985 Nov 20;186(2):283-93 PMID: 3910840
  13. Unique self-complementarity of palindromic sequences provides DNA structural intermediates for mutation.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:851-61 PMID: 6345080
  14. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  15. Repair of DNA base-pair mismatches in extracts of Escherichia coli.
    Cold Spring Harb Symp Quant Biol. 1984;49:589-96 PMID: 6397313
  16. Mutational specificity of depurination.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1494-8 PMID: 6369329
  17. Sequence of the lacI gene.
    Nature. 1978 Aug 24;274(5673):765-9 PMID: 355891
  18. Sites of reaction of Escherichia coli DNA gyrase on pBR322 in vivo as revealed by oxolinic acid-induced plasmid linearization.
    J Mol Biol. 1985 Jan 5;181(1):63-74 PMID: 2984430
  19. The dnaA protein complex with the E. coli chromosomal replication origin (oriC) and other DNA sites.
    Cell. 1984 Oct;38(3):889-900 PMID: 6091903
  20. Structural intermediates of deletion mutagenesis: a role for palindromic DNA.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):512-6 PMID: 6582506
  21. On the role of DNA polymerase in base selection.
    Cold Spring Harb Symp Quant Biol. 1966;31:693-7 PMID: 5237208
  22. Spontaneous mutagenesis: the roles of DNA repair, replication, and recombination.
    Mutat Res. 1985 Jul;154(1):1-27 PMID: 3889622
  23. Rapid repeated cloning of mutant lac repressor genes.
    Gene. 1985;39(2-3):181-9 PMID: 4092929
  24. Genetic control of mutation rates in bacteriophageT4.
    Nature. 1969 Mar 22;221(5186):1128-32 PMID: 4975273
  25. Isolation of an Escherichia coli K-12 dnaE mutation as a mutator.
    J Bacteriol. 1978 Mar;133(3):1197-202 PMID: 346561
  26. Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma.
    Cell. 1984 Dec;39(3 Pt 2):653-62 PMID: 6096017
  27. Frameshift mutations and the genetic code. This paper is dedicated to Professor Theodosius Dobzhansky on the occasion of his 66th birthday.
    Cold Spring Harb Symp Quant Biol. 1966;31:77-84 PMID: 5237214
  28. Isolation of an E. coli strain with a mutation affecting DNA polymerase.
    Nature. 1969 Dec 20;224(5225):1164-6 PMID: 4902142
  29. On the formation of spontaneous deletions: the importance of short sequence homologies in the generation of large deletions.
    Cell. 1982 Jun;29(2):319-28 PMID: 6288254
  30. Induced mutagenesis in dam- mutants of Escherichia coli: a role for 6-methyladenine residues in mutation avoidance.
    Mol Gen Genet. 1978 Jul 25;163(3):307-12 PMID: 355857
  31. Mechanisms of spontaneous mutagenesis: clues from altered mutational specificity in DNA repair-defective strains.
    Basic Life Sci. 1986;39:259-81 PMID: 3021113
  32. Genetic analysis of an E. coli strain with a mutation affecting DNA polymerase.
    Nature. 1969 Dec 20;224(5225):1166-8 PMID: 4902143
  33. Mechanisms of spontaneous mutagenesis: an analysis of the spectrum of spontaneous mutation in the Escherichia coli lacI gene.
    J Mol Biol. 1986 May 20;189(2):273-84 PMID: 3018259
  34. Chi hotspots of generalized recombination.
    Cell. 1983 Oct;34(3):709-10 PMID: 6354468
  35. Characterization of mutational specificity within the lacI gene for a mutD5 mutator strain of Escherichia coli defective in 3'----5' exonuclease (proofreading) activity.
    J Bacteriol. 1986 Jul;167(1):130-7 PMID: 3522541
  36. Increased spontaneous reversion of certain frameshift mutations in DNA polymerase I deficient strains of Escherichia coli.
    Mol Gen Genet. 1975 Dec 1;141(3):251-62 PMID: 1107818
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331588
Published
1987-05-00
Pages
267-72
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00331588
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