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PMID: 3298217 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Purification and characterization of the CheZ protein of bacterial chemotaxis.

Journal of bacteriology ·Vol. 169 ·No. 7 ·1987-07-00 ·Pages 3301-11

Stock AM, Stock JB

Abstract

The cheZ gene is the most distal of five genes that comprise the Meche operon of the Salmonella typhimurium chemotaxis system. We have determined the sequence of the cheZ gene along with an 800-nucleotide flanking region at its 3' end. The flanking sequence contains an open reading frame that probably corresponds to the 5' end of flaM. The cheZ coding sequence predicts an extremely acidic, hydrophilic protein with a molecular weight of 23,900. We have purified and characterized this protein. N-terminal analysis of pure CheZ yields an amino acid sequence identical to that predicted by the nucleotide sequence except that the amino-terminal methionine residue is modified by N methylation. The purified CheZ protein exhibits a native molecular weight of 115,000, but in cell extracts the majority of CheZ exists as a much larger aggregate (Mr greater than 500,000). Under these conditions, CheZ appears to be a homopolymer composed of at least 20 monomeric subunits.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,immunology,isolation & purification Base Sequence Carboxylic Ester Hydrolases/metabolism Chemotaxis Chromosome Mapping Genes, Bacterial Immunologic Techniques Macromolecular Substances Membrane Proteins/isolation & purification Methyl-Accepting Chemotaxis Proteins Molecular Weight Operon Protein Binding Salmonella typhimurium/analysis Solubility
Chemicals
Bacterial Proteins Macromolecular Substances Membrane Proteins Methyl-Accepting Chemotaxis Proteins Carboxylic Ester Hydrolases chemotactic protein methylesterase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stock A M
Stock J B
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.7.3301-3311.1987
Published
1987-07-00
Pages
3301-11
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212384
Subset
IM
Grants
NIAID NIH HHS · AI20980 · United States
Databases
GENBANK
M16691
External Links
PubMed source DOI: 10.1128/jb.169.7.3301-3311.1987 PMC Full Text View journal details
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