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PMID: 3283738 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An alternative approach to somatic cell gene therapy.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 9 ·1988-05-00 ·Pages 3150-4

St Louis D, Verma IM

Abstract

Mouse primary skin fibroblasts were infected with a recombinant retrovirus containing human factor IX cDNA. Bulk infected cells capable of synthesizing and secreting biologically active human factor IX protein were embedded in collagen, and the implant was grafted under the epidermis. Sera from the transplanted mice contain human factor IX protein for at least 10-12 days. Loss of immunoreactive human factor IX protein in the mouse serum is not due to graft rejection. Instead, the mouse serum contains anti-human factor IX antibodies, which react with the protein. We suggest that retroviral-infected primary skin fibroblasts offer an alternative approach to somatic cell gene therapy.

MeSH Terms
Animals Antibodies/analysis DNA/analysis Drug Resistance, Microbial Factor IX/genetics,metabolism Fibroblasts/transplantation Gene Expression Regulation Genetic Engineering/methods Humans Mice Mice, Inbred C57BL Neomycin/pharmacology Retroviridae/genetics Skin/cytology Skin Transplantation Transfection
Chemicals
Antibodies Factor IX DNA Neomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
St Louis D
Molecular Biology and Virology Laboratory, Salk Institute, San Diego, CA 92138.
Verma I M
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.9.3150
Published
1988-05-00
Pages
3150-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280161
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.9.3150 PMC Full Text View journal details
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