Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 3 ·1979-03-00 ·Pages 1274-8

Bell E, Ivarsson B, Merrill C

Abstract

Fibroblasts can condense a hydrated collagen lattice to a tissue-like structure 1/28th the area of the starting gel in 24 hr. The rate of the process can be regulated by varying the protein content of the lattice, the cell number, or the concentration of an inhibitor such as Colcemid. Fibroblasts of high population doubling level propagated in vitro, which have left the cell cycle, can carry out the contraction at least as efficiently as cycling cells. The potential uses of the system as an immunologically tolerated "tissue" for wound healing and as a model for studying fibroblast function are discussed.

MeSH Terms

Animals Cell Line Collagen/metabolism Cytarabine/pharmacology Cytochalasin B/pharmacology Demecolcine/pharmacology Fibroblasts/drug effects,metabolism Humans Kinetics Male Protein Conformation Rats Skin

Chemicals

Cytarabine Cytochalasin B Collagen Demecolcine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bell E

Ivarsson B

Merrill C

References (10)

10 references, click to expand

The growth of cells on a transparent gel of reconstituted rat-tail collagen.
J Natl Cancer Inst. 1956 Jun;16(6):1375-403 PMID: 13320119
Studies in wound healing. III. Contraction in vit. C. deficiency.
Proc Soc Exp Biol Med. 1959 Jun;101(2):268-70 PMID: 13675208
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Hormonal effects on intracellular and secreted casein in cultures of mouse mammary epithelial cells on floating collagen membranes.
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4466-70 PMID: 270692
Granulation tissue as a contractile organ. A study of structure and function.
J Exp Med. 1972 Apr 1;135(4):719-34 PMID: 4336123
Collagen substrata for studies on cell behavior.
J Cell Biol. 1972 Sep;54(3):626-37 PMID: 4339818
Contraction of granulation tissue in vitro: similarity to smooth muscle.
Science. 1971 Aug 6;173(3996):548-50 PMID: 4327529
The fibroblast and wound repair.
Biol Rev Camb Philos Soc. 1968 Feb;43(1):51-96 PMID: 4229841
Cellular senescence and DNA synthesis. Thymidine incorporation as a measure of population age in human diploid cells.
Exp Cell Res. 1973 Feb;76(2):419-27 PMID: 4568162
Cytochalasin effects on BALB-3T3 fibroblasts: dose-dependent, reversible alteration of motility and cytoplasmic cleavage.
Exp Cell Res. 1971 Sep;68(1):226-8 PMID: 5165445

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.76.3.1274

Published

1979-03-00

Pages

1274-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC383233

Subset

External Links

PubMed source DOI: 10.1073/pnas.76.3.1274 PMC Full Text View journal details

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Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro.

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