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PMID: 3282081 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Simian retrovirus-D serotype 1 (SRV-1) envelope glycoproteins gp70 and gp20: expression in yeast cells and identification of specific antibodies in sera from monkeys that recovered from SRV-1 infection.

Journal of virology ·Vol. 62 ·No. 5 ·1988-05-00 ·Pages 1774-80

Kwang HS, Barr PJ, Sabin EA, Sujipto S, Marx PA, Power MD, Bathurst IC, Pedersen NC

Abstract

The gp70 and transmembrane gp20 envelope proteins of simian retrovirus-D serotype 1 (SRV-1) were expressed in Saccharomyces cerevisiae as fusion proteins with human superoxide dismutase (SOD). Expression of the SOD-gp70 and SOD-gp20 sequences yielded fusion proteins of 52 and 29 kilodaltons, respectively. The yeast-expressed SRV-1 envelope proteins were used in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in the sera of rhesus macaques that recovered from SRV-1. Sera from 47 of 49 such monkeys tested positive for antibodies to the SOD-gp70 fusion protein, while 45 of 49 reacted positively to SOD-gp20. None of 26 SRV-1-nonexposed monkeys tested positive in either ELISA. Monkeys immunized with the recombinant SRV-1 gp20 and gp70 proteins made good ELISA and Western blot (immunoblot) antibodies to whole SRV-1. This antibody was not neutralizing in vitro, however.

MeSH Terms

Acquired Immunodeficiency Syndrome/veterinary Animals Antibodies, Viral/analysis Enzyme-Linked Immunosorbent Assay Gene Expression Regulation Macaca mulatta Monkey Diseases/microbiology Plasmids Recombinant Fusion Proteins/biosynthesis Retroviridae/immunology Retroviridae Proteins/biosynthesis,immunology Saccharomyces cerevisiae/genetics,metabolism Viral Envelope Proteins/biosynthesis,immunology

Chemicals

Antibodies, Viral Recombinant Fusion Proteins Retroviridae Proteins Viral Envelope Proteins

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Kwang H S

Department of Medicine, School of Veterinary Medicine, University of California, Davis 95616.

Barr P J

Sabin E A

Sujipto S

Marx P A

Power M D

Bathurst I C

Pedersen N C

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.62.5.1774-1780.1988

Published

1988-05-00

Pages

1774-80

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC253226

Subset

Grants

PHS HHS · A124292-0151 · United States

PHS HHS · R0I-A120573-01 · United States

NCRR NIH HHS · RR00169 · United States

External Links

PubMed source DOI: 10.1128/JVI.62.5.1774-1780.1988 PMC Full Text View journal details

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Simian retrovirus-D serotype 1 (SRV-1) envelope glycoproteins gp70 and gp20: expression in yeast cells and identification of specific antibodies in sera from monkeys that recovered from SRV-1 infection.

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