Home LiteratureArticle Details
PMID: 3278196 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Enhancement of copper resistance and CupI amplification in carcinogen-treated yeast cells.

Molecular & general genetics : MGG ·Vol. 211 ·No. 1 ·1988-01-00 ·Pages 88-94

Aladjem MI, Koltin Y, Lavi S

Abstract

Carcinogen-induced amplification at the CupI locus, coding for a metallothionein protein, was studied in the yeast Saccharomyces cerevisiae. Exposure of cells from three different haploid strains, 4939, DBY746 and 320, to chemical carcinogens such as N-methyl-N'-nitro-N-nitroso-guanidine (MNNG), ethylmethanesulfonate (EMS) and 4-nitroquinoline-N-oxide (4NQO) enhanced the frequency of copper-resistant colonies up to several hundred fold. Copper-resistant clones obtained from strains DBY746 and 320, which contain more than one copy of the CupI locus, displayed a four- to eightfold amplification of the CupI sequences. In these clones the amplified CupI sequences were organized in a tandem array. Carcinogen treatment of strain 4939 in which only one copy of the CupI gene is present produced resistant colonies without CupI amplification. The possible use of the yeast system to study gene duplication and amplification is discussed.

MeSH Terms
Carcinogens/toxicity Chromosome Mapping Copper/toxicity Drug Resistance Gene Amplification/drug effects Genes, Fungal Metallothionein/genetics Mutation/drug effects Saccharomyces cerevisiae/genetics
Chemicals
Carcinogens Copper Metallothionein
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Aladjem M I
Department of Microbiology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat-Aviv, Israel.
Koltin Y
Lavi S
References (29)
29 references, click to expand
  1. Tandem gene amplification mediates copper resistance in yeast.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5342-6 PMID: 6291039
  2. Carcinogen-mediated induction of SV40 DNA synthesis in SV40 transformed Chinese hamster embryo cells.
    Carcinogenesis. 1981;2(5):417-23 PMID: 6268326
  3. Homogeneously staining chromosomal regions contain amplified copies of an abundantly expressed cellular oncogene (c-myc) in malignant neuroendocrine cells from a human colon carcinoma.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1707-11 PMID: 6300869
  4. Rapid spontaneous dihydrofolate reductase gene amplification shown by fluorescence-activated cell sorting.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3711-5 PMID: 6574509
  5. Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo.
    Cell. 1983 May;33(1):211-9 PMID: 6380751
  6. Unstable DNA amplifications in methotrexate-resistant Leishmania consist of extrachromosomal circles which relocalize during stabilization.
    Cell. 1984 Sep;38(2):431-9 PMID: 6467372
  7. Carcinogen-mediated methotrexate resistance and dihydrofolate reductase amplification in Chinese hamster cells.
    Mol Cell Biol. 1986 Jun;6(6):1958-64 PMID: 3023913
  8. Gene amplification, drug resistance, and cancer.
    Cancer Res. 1984 May;44(5):1735-42 PMID: 6713376
  9. A cellular oncogene (c-Ki-ras) is amplified, overexpressed, and located within karyotypic abnormalities in mouse adrenocortical tumour cells.
    Nature. 1983 Jun 9-15;303(5917):497-501 PMID: 6304530
  10. UV radiation facilitates methotrexate resistance and amplification of the dihydrofolate reductase gene in cultured 3T6 mouse cells.
    Mol Cell Biol. 1984 Jun;4(6):1050-6 PMID: 6738530
  11. Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
    Science. 1968 Apr 19;160(3825):272-80 PMID: 4867987
  12. Resistance to antimycin A in yeast by amplification of ADH4 on a linear, 42 kb palindromic plasmid.
    Cell. 1986 Sep 12;46(6):857-63 PMID: 3019553
  13. Carcinogen-mediated amplification of viral DNA sequences in simian virus 40-transformed Chinese hamster embryo cells.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6144-8 PMID: 6273853
  14. Meiotic gene conversion mediates gene amplification in yeast.
    Cold Spring Harb Symp Quant Biol. 1984;49:55-65 PMID: 6397309
  15. Function and autoregulation of yeast copperthionein.
    Science. 1985 May 10;228(4700):685-90 PMID: 3887570
  16. Tandem genetic duplications in phage and bacteria.
    Annu Rev Microbiol. 1977;31:473-505 PMID: 334045
  17. Cloning of a yeast dihydrofolate reductase gene in Escherichia coli.
    Curr Genet. 1984 May;8(4):265-70 PMID: 24177794
  18. Amplification of endogenous myc-related DNA sequences in a human myeloid leukaemia cell line.
    Nature. 1982 Aug 12;298(5875):679-81 PMID: 6285209
  19. A product of yeast RAS2 gene is a guanine nucleotide binding protein.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6924-8 PMID: 6438624
  20. ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae.
    Nature. 1983 Dec 15-21;306(5944):707-9 PMID: 6318116
  21. Alkylation of DNA and tissue specificity in nitrosamine carcinogenesis.
    J Supramol Struct Cell Biochem. 1981;17(3):259-73 PMID: 7328674
  22. A yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product.
    Nature. 1983 Dec 15-21;306(5944):704-7 PMID: 6318115
  23. Primary structure and transcription of an amplified genetic locus: the CUP1 locus of yeast.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):337-41 PMID: 6364141
  24. Amplification of genes for chorion proteins during oogenesis in Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):1096-100 PMID: 6767241
  25. Gene amplification.
    Annu Rev Biochem. 1984;53:447-91 PMID: 6383198
  26. Phorbol ester dramatically increases incidence of methotrexate-resistant mouse cells: possible mechanisms and relevance to tumor promotion.
    Cell. 1981 Aug;25(2):561-72 PMID: 7285113
  27. Replication intermediates formed during initiation of DNA synthesis in methotrexate-resistant CHOC 400 cells are enriched for sequences derived from a specific, amplified restriction fragment.
    Biochemistry. 1986 Jan 28;25(2):441-9 PMID: 3006761
  28. Evidence that induction and suppression of mutations and recombinations by chemical mutagens in S. cerevisiae during mitosis are jointly correlated.
    Mol Gen Genet. 1979 Jan 10;168(2):125-39 PMID: 109736
  29. Industrial yeasts display tandem gene iteration at the CUP1 region.
    Mol Cell Biol. 1983 Aug;3(8):1353-61 PMID: 6621529
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00338397
Published
1988-01-00
Pages
88-94
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00338397
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2