Home LiteratureArticle Details
PMID: 3275632 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the iucD gene of the pColV-K30 aerobactin operon and topology of its product studied with phoA and lacZ gene fusions.

Journal of bacteriology ·Vol. 170 ·No. 1 ·1988-01-00 ·Pages 56-64

Herrero M, de Lorenzo V, Neilands JB

Abstract

Gene iucD of the aerobactin operon of the Escherichia coli plasmid ColV-K30 encodes a membrane-bound enzyme synthesizing N6-hydroxylysine, the first product of the aerobactin biosynthesis pathway. The entire nucleotide sequence of the cloned iucD gene was determined, from which the primary and some aspects of the secondary structure of the encoded peptide were deduced. E. coli cells harboring multicopy plasmid pVLN12 (iucD+) hyperproduced an approximately 50-kilodalton peptide which was purified and identified as the product of the gene by examination of its amino-terminal sequence. Two iucD'-'lacZ gene fusions were constructed in vitro and four iucD'-'phoA gene fusions were generated in vivo by mutagenesis of iucD with transposon TnphoA (Tn5 IS50L::phoA). Analysis of the corresponding fusion proteins suggested at least two domains of attachment of the IucD protein to the inner side of the cytoplasmic membrane. The first apparent membrane-bound domain was found within the first 25 amino acids of the protein and showed a sequence which resembled that of the signal peptides.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,isolation & purification Base Sequence Cloning, Molecular DNA, Bacterial/genetics Escherichia coli/genetics Genes, Bacterial Hydroxamic Acids/biosynthesis Iron Chelating Agents Mixed Function Oxygenases Molecular Sequence Data Operon Plasmids Recombinant Fusion Proteins/analysis,genetics
Chemicals
Bacterial Proteins DNA, Bacterial Hydroxamic Acids Iron Chelating Agents Recombinant Fusion Proteins aerobactin Mixed Function Oxygenases lysine N-epsilon hydroxylase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Herrero M
Department of Biochemistry, University of California, Berkeley 94720.
de Lorenzo V
Neilands J B
References (42)
42 references, click to expand
  1. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  2. Conversion of beta-galactosidase to a membrane-bound state by gene fusion.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3423-7 PMID: 790385
  3. Protein localization in E. coli: is there a common step in the secretion of periplasmic and outer-membrane proteins?
    Cell. 1981 Jun;24(3):707-17 PMID: 6788377
  4. Regulation of ferric iron transport in Escherichia coli K12: isolation of a constitutive mutant.
    Mol Gen Genet. 1981;182(2):288-92 PMID: 7026976
  5. Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes.
    Gene. 1982 Jun;18(3):199-209 PMID: 6751939
  6. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  7. Cloning and expression of the cloacin DF13/aerobactin receptor of Escherichia coli (ColV-K30).
    J Bacteriol. 1983 Feb;153(2):716-21 PMID: 6218156
  8. Structure of the serine chemoreceptor in Escherichia coli.
    Nature. 1983 Feb 17-23;301(5901):623-6 PMID: 6402709
  9. Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli.
    J Bacteriol. 1983 Apr;154(1):366-74 PMID: 6339478
  10. Separation of signal transduction and adaptation functions of the aspartate receptor in bacterial sensing.
    Science. 1983 Jun 3;220(4601):1016-20 PMID: 6302843
  11. Iron absorption and transport in microorganisms.
    Annu Rev Nutr. 1981;1:27-46 PMID: 6226306
  12. Subcloning of the cloacin DF13/aerobactin receptor protein and identification of a pColV-K30-determined polypeptide involved in ferric-aerobactin uptake.
    J Bacteriol. 1983 Nov;156(2):945-8 PMID: 6313629
  13. Sequence homology between the tetracycline-resistance determinants of Tn10 and pBR322.
    Gene. 1983 Nov;25(1):83-92 PMID: 6319234
  14. A cluster of five genes specifying the aerobactin iron uptake system of plasmid ColV-K30.
    Infect Immun. 1984 Oct;46(1):7-12 PMID: 6090318
  15. Genetic and biochemical characterization of the aerobactin synthesis operon on pColV.
    Mol Gen Genet. 1984;196(1):74-80 PMID: 6434902
  16. The relationship of plasmid-mediated iron transport and bacterial virulence.
    Annu Rev Microbiol. 1984;38:69-89 PMID: 6093687
  17. Simple, rapid, and quantitative release of periplasmic proteins by chloroform.
    J Bacteriol. 1984 Dec;160(3):1181-3 PMID: 6501229
  18. Mapping of a mutation affecting regulation of iron uptake systems in Escherichia coli K-12.
    J Bacteriol. 1985 Jan;161(1):450-3 PMID: 3918009
  19. Fine structure of a membrane anchor domain.
    J Mol Biol. 1985 Jan 5;181(1):111-21 PMID: 3872373
  20. Promoter mapping and transcriptional regulation of the iron assimilation system of plasmid ColV-K30 in Escherichia coli K-12.
    J Bacteriol. 1985 Jun;162(3):1039-46 PMID: 2581932
  21. Failure of E. coli K-12 to transport PhoE-LacZ hybrid proteins out of the cytoplasm.
    EMBO J. 1985 Apr;4(4):1041-7 PMID: 2990905
  22. Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):5107-11 PMID: 3860846
  23. Rare codons in E. coli and S. typhimurium signal sequences.
    FEBS Lett. 1985 Sep 23;189(2):318-24 PMID: 3899723
  24. TnphoA: a transposon probe for protein export signals.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):8129-33 PMID: 2999794
  25. Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12.
    J Bacteriol. 1986 Feb;165(2):570-8 PMID: 2935523
  26. Identification of the genes and their polypeptide products responsible for aerobactin synthesis by pColV plasmids.
    Mol Gen Genet. 1985;201(2):204-12 PMID: 3003525
  27. Isolation and properties of N epsilon-hydroxylysine:acetyl coenzyme A N epsilon-transacetylase from Escherichia coli pABN11.
    Biochemistry. 1986 May 6;25(9):2485-9 PMID: 3521734
  28. Electroelution of fixed and stained membrane proteins from preparative sodium dodecyl sulfate-polyacrylamide gels into a membrane trap.
    Anal Biochem. 1986 May 1;154(2):583-9 PMID: 2425657
  29. RNA-DNA hybridization analysis of transcription of the plasmid ColV-K30 aerobactin gene cluster.
    J Bacteriol. 1986 Aug;167(2):467-72 PMID: 3015872
  30. Use of TnphoA to detect genes for exported proteins in Escherichia coli: identification of the plasmid-encoded gene for a periplasmic acid phosphatase.
    J Bacteriol. 1987 Apr;169(4):1663-9 PMID: 3031017
  31. A genetic approach to analyzing membrane protein topology.
    Science. 1986 Sep 26;233(4771):1403-8 PMID: 3529391
  32. Effect of distribution of unfavourable codons on the maximum rate of gene expression by an heterologous organism.
    J Theor Biol. 1986 May 7;120(1):99-110 PMID: 3528671
  33. Gene expression in Zymomonas mobilis: promoter structure and identification of membrane anchor sequences forming functional lacZ' fusion proteins.
    J Bacteriol. 1987 Jun;169(6):2327-35 PMID: 3034853
  34. Export of unprocessed precursor maltose-binding protein to the periplasm of Escherichia coli cells.
    J Bacteriol. 1987 Jun;169(6):2352-9 PMID: 3294787
  35. Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation (fur) repressor.
    J Bacteriol. 1987 Jun;169(6):2624-30 PMID: 3294800
  36. The use of transposon TnphoA to detect genes for cell envelope proteins subject to a common regulatory stimulus. Analysis of osmotically regulated genes in Escherichia coli.
    J Mol Biol. 1987 May 20;195(2):289-97 PMID: 2821274
  37. Ferric uptake regulation protein acts as a repressor, employing iron (II) as a cofactor to bind the operator of an iron transport operon in Escherichia coli.
    Biochemistry. 1987 Aug 25;26(17):5471-7 PMID: 2823881
  38. Molecular mechanism of regulation of siderophore-mediated iron assimilation.
    Microbiol Rev. 1987 Dec;51(4):509-18 PMID: 2963952
  39. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  40. Determinant of cistron specificity in bacterial ribosomes.
    Nature. 1975 Mar 6;254(5495):34-8 PMID: 803646
  41. Genetic analysis of regulatory mutants of alkaline phosphatase of E. coli.
    Genetics. 1975 Nov;81(3):459-68 PMID: 1107145
  42. Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm.
    J Bacteriol. 1979 Jul;139(1):19-31 PMID: 110778
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.1.56-64.1988
Published
1988-01-00
Pages
56-64
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210605
Subset
IM
Grants
FIC NIH HHS · 1F05TW03577-01-BI-5 · United States
PHS HHS · A104156 · United States
Databases
GENBANK
M18968
External Links
PubMed source DOI: 10.1128/jb.170.1.56-64.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2