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PMID: 3267219 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of mutant monoclonal antibodies with increased antigen binding.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 7 ·1988-04-00 ·Pages 2298-302

Pollock RR, French DL, Gefter ML, Scharff MD

Abstract

Sib selection and an ELISA have been used to isolate hybridoma subclones producing mutant antibodies that bind antigen better than the parental monoclonal antibody. Such mutants arise spontaneously in culture at frequencies of 2.5-5 X 10(-5). The sequences of the heavy and light chain variable regions of the mutant antibodies are identical to that of the parent and the Ka values of the mutants and the parent are the same. The increase in binding is associated with abnormalities of the constant region polypeptide and probably reflect changes in avidity of these antibodies.

MeSH Terms
Animals Antibodies, Monoclonal/genetics,immunology,metabolism Antigen-Antibody Reactions Antigens/metabolism Base Sequence Enzyme-Linked Immunosorbent Assay Hybridomas/immunology Immunoglobulin G/immunology Immunoglobulin Variable Region/genetics Mice Protein Binding
Chemicals
Antibodies, Monoclonal Antigens Immunoglobulin G Immunoglobulin Variable Region
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pollock R R
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461.
French D L
Gefter M L
Scharff M D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.7.2298
Published
1988-04-00
Pages
2298-302
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279978
Subset
IM
Grants
NIAID NIH HHS · AI05231 · United States
NIAID NIH HHS · AI10702 · United States
NCI NIH HHS · CA39838 · United States
Databases
GENBANK
M20274, M20275, M20276, M20277, M20278, M20279, M20280, M20281
External Links
PubMed source DOI: 10.1073/pnas.85.7.2298 PMC Full Text View journal details
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