Interactions of radio-detoxified Escherichia coli endotoxin preparations with the complement system.

Infection and immunity ·Vol. 16 ·No. 1 ·1977-04-00 ·Pages 26-31

Füst G, Bertók L, Juhász-Nagy S

Abstract

Escherichia coli O89 lipopolysaccharide (LPS) was treated with different doses of gamma irradiation (5, 10, 15, and 20 Mrad). Various biological activities such as lethal effect, decrease in arterial blood pressure in dogs, and interaction with the complement system were determined for the parent and irradiated preparations. Irradiation of LPS significantly and in a dose-dependent manner decreased its lethal and blood pressure-depressing effects along with its ability to activate the complement system. In contrast, radio-detoxified LPS fixed more strongly the isolated human C1 than did the parent LPS. The possible connection between the toxicity of endotoxin and endotoxin-induced complement activation is discussed.

MeSH Terms

Animals Blood Pressure Complement C1/metabolism Complement C3/metabolism Complement C4/metabolism Complement System Proteins/metabolism Dogs Dose-Response Relationship, Radiation Endotoxins/radiation effects Escherichia coli Female Gamma Rays Guinea Pigs Humans In Vitro Techniques Lethal Dose 50 Lipopolysaccharides/radiation effects Male Polysaccharides, Bacterial/radiation effects Rats

Chemicals

Complement C1 Complement C3 Complement C4 Endotoxins Lipopolysaccharides Polysaccharides, Bacterial Complement System Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Füst G

Bertók L

Juhász-Nagy S

References (24)

24 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.16.1.26-31.1977

Published

1977-04-00

Pages

26-31

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC421482

Subset

External Links

PubMed source DOI: 10.1128/iai.16.1.26-31.1977 PMC Full Text View journal details

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Interactions of radio-detoxified Escherichia coli endotoxin preparations with the complement system.

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