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PMID: 1204244 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The effect of anticomplementary substances on properdin in normal and C2-deficient sera.

Clinical and experimental immunology ·Vol. 19 ·No. 3 ·1975-03-00 ·Pages 435-44

McLean RH, Townsend K, Michael AF

Abstract

The effect of anticomplementary substances, including zymosan, cobra venom, endotoxin, inulin and immune complexes, on serum properdin concentration and immunoelectrophoretic mobility was studied. In normal serum, zymosan, inulin and immune complexes 'fixed' properdin, while in C2-deficient serum, only zymyosan 'fixed' properdin. Slowly migrating properdin (P) was detected in normal serum following activation by endotoxin and cobra venom but in C2-deficient serum only with cobra venom. Endotoxin did not activate the alternative pathway proteins studied in C2-deficient serum. Fast migrating properdin (P) represented activated properdin and occured as a result of activation of properdin in the Noble agar medium used for electrophoresis provided sufficient cofactors, including Mg2+, were present.

MeSH Terms
Antigen-Antibody Complex Complement C2/deficiency Complement C3/analysis Complement Inactivator Proteins Complement System Proteins/deficiency Endotoxins/pharmacology Humans Immunoelectrophoresis Inulin/pharmacology Properdin/analysis Snake Venoms/pharmacology Zymosan/pharmacology
Chemicals
Antigen-Antibody Complex Complement C2 Complement C3 Complement Inactivator Proteins Endotoxins Snake Venoms Properdin Inulin Complement System Proteins Zymosan
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McLean R H
Townsend K
Michael A F
References (19)
19 references, click to expand
  1. C3 proactivator convertase and its mode of action.
    J Exp Med. 1972 Apr 1;135(4):1003-8 PMID: 4111773
  2. The properdin system and immunity. III. The zymosan assay of properdin.
    J Exp Med. 1956 Jan 1;103(1):1-13 PMID: 13278451
  3. The utilization of properdin in the alternate pathway of complement activation: isolation of properdin convertase.
    J Immunol. 1974 Jan;112(1):56-62 PMID: 4130153
  4. Properties of highly purified human properdin.
    J Immunol. 1968 Jan;100(1):142-58 PMID: 4169693
  5. Interactions of the complement system with endotoxic lipopolysaccharide: consumption of each of the six terminal complement components.
    J Exp Med. 1968 Nov 1;128(5):1049-57 PMID: 4176223
  6. Opsonic activity in human serum deficient in C2.
    J Immunol. 1972 Jul;109(1):141-5 PMID: 4402482
  7. C2 deficiency. Development of lupus erythematosus.
    J Clin Invest. 1973 Jul;52(7):1601-7 PMID: 4578155
  8. The immunoelectrophoretic pattern of properdin in fresh and aged human serum.
    Proc Soc Exp Biol Med. 1972 Nov;141(2):403-7 PMID: 4629085
  9. Properdin anc C3 proactivator: alternate pathway components in human glomerulonephritis.
    J Clin Invest. 1973 Mar;52(3):634-44 PMID: 4630981
  10. Determination of serum properdin levels by immunodiffusion.
    Int Arch Allergy Appl Immunol. 1973;44(1):155-60 PMID: 4633166
  11. Interactions of the classical and alternate complement pathway with endotoxin lipopolysaccharide. Effect on platelets and blood coagulation.
    J Clin Invest. 1973 Feb;52(2):370-6 PMID: 4683877
  12. The reaction of zymosan with the properdin system in normal and C4-deficienct guinea pig serum. Demonstration of C3- and C5-cleaving multi-unit enzymes, both containing factor B, and acceleration of their formation by the classical complement pathway.
    J Immunol. 1973 Nov;111(5):1389-400 PMID: 4795621
  13. The role of properdin in the alternate pathway of complement activation.
    J Exp Med. 1974 Jan 1;139(1):44-57 PMID: 4808709
  14. The complement system in chronic glomerulonephritis: three newly associated aberrations.
    J Pediatr. 1971 Jan;78(1):30-43 PMID: 4992579
  15. Effect of C4 depletion on the utilization of the terminal components of guinea-pig complement by endotoxin.
    Nat New Biol. 1971 Jun 2;231(22):152-4 PMID: 4997056
  16. Isolation of the anticomplementary protein from cobra venom and its mode of action on C3.
    J Immunol. 1971 Dec;107(6):1666-72 PMID: 5120401
  17. An alternate complement pathway: C-3 cleaving activity, not due to C4,2a, on endotoxic lipopolysaccharide after treatment with guinea pig serum; relation to properdin.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1351-4 PMID: 5288385
  18. The properdin system and immunity. I. Demonstration and isolation of a new serum protein, properdin, and its role in immune phenomena.
    Science. 1954 Aug 20;120(3112):279-85 PMID: 13186838
  19. Activation of complement by endotoxin: a role for 2 globulin, C1, C4 and C2 in the consumption of terminal complement components by endotoxin-coated erythrocytes.
    J Immunol. 1972 Aug;109(2):334-41 PMID: 4114826
Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
Published
1975-03-00
Pages
435-44
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1538159
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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