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PMID: 3264556 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Amino acid sequence and domain structure of entactin. Homology with epidermal growth factor precursor and low density lipoprotein receptor.

The Journal of cell biology ·Vol. 107 ·No. 6 Pt 2 ·1988-12-00 ·Pages 2749-56

Durkin ME, Chakravarti S, Bartos BB, Liu SH, Friedman RL, Chung AE

Abstract

Entactin (nidogen), a 150-kD sulfated glycoprotein, is a major component of basement membranes and forms a highly stable noncovalent complex with laminin. The complete amino acid sequence of mouse entactin has been derived from sequencing of cDNA clones. The 5.9-kb cDNA contains a 3,735-bp open reading frame followed by a 3'-untranslated region of 2.2 kb. The open reading frame encodes a 1,245-residue polypeptide with an unglycosylated Mr of 136,500, a 28-residue signal peptide, two Asn-linked glycosylation sites, and two potential Ca2+-binding sites. Analysis of the deduced amino acid sequence predicts that the molecule consists of two globular domains of 70 and 36 kD separated by a cysteine-rich domain of 28 kD. The COOH-terminal globular domain shows homology to the EGF precursor and the low density lipoprotein receptor. Entactin contains six EGF-type cysteine-rich repeat units and one copy of a cysteine-repeat motif found in thyroglobulin. The Arg-Gly-Asp cell recognition sequence is present in one of the EGF-type repeats, and a synthetic peptide from the putative cell-binding site of entactin was found to promote the attachment of mouse mammary tumor cells.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Basement Membrane/analysis Cell Adhesion Cell Line Cloning, Molecular DNA/genetics Epidermal Growth Factor/genetics Glycoproteins/analysis,genetics Membrane Glycoproteins Mice Molecular Sequence Data Protein Precursors/genetics Receptors, LDL/genetics Sequence Homology, Nucleic Acid
Chemicals
Glycoproteins Membrane Glycoproteins Protein Precursors Receptors, LDL epidermal growth factor precursor nidogen Epidermal Growth Factor DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Durkin M E
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260.
Chakravarti S
Bartos B B
Liu S H
Friedman R L
Chung A E
References (53)
53 references, click to expand
  1. The role of beta-hydroxyaspartate and adjacent carboxylate residues in the first EGF domain of human factor IX.
    EMBO J. 1988 Jul;7(7):2053-61 PMID: 3262057
  2. The role of Ca2+ binding in the self-aggregation of laminin-nidogen complexes.
    J Biol Chem. 1988 Apr 15;263(11):5425-30 PMID: 3128539
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
    J Mol Biol. 1978 Mar 25;120(1):97-120 PMID: 642007
  6. Properties of a basement membrane-related glycoprotein synthesized in culture by a mouse embryonal carcinoma-derived cell line.
    Cell. 1979 Feb;16(2):277-87 PMID: 88263
  7. Epidermal growth factor.
    Annu Rev Biochem. 1979;48:193-216 PMID: 382984
  8. Structure and evolution of calcium-modulated proteins.
    CRC Crit Rev Biochem. 1980;8(2):119-74 PMID: 6105043
  9. Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.
    Anal Biochem. 1980 Nov 15;109(1):76-86 PMID: 6258458
  10. Entactin, a novel basal lamina-associated sulfated glycoprotein.
    J Biol Chem. 1981 May 25;256(10):5209-14 PMID: 6262321
  11. Immunolocalization of entactin, a sulfated basement membrane component, in rodent tissues, and comparison with GP-2 (laminin).
    Am J Pathol. 1981 Jun;103(3):419-26 PMID: 6165248
  12. Transmembrane assembly of membrane and secretory glycoproteins.
    Arch Biochem Biophys. 1981 Oct 1;211(1):1-19 PMID: 7030209
  13. Murine parietal endoderm cells synthesise heparan sulphate and 170K and 145K sulphated glycoproteins as components of Reichert's membrane.
    Dev Biol. 1982 Mar;90(1):210-4 PMID: 6460654
  14. Synthesis and localization of two sulphated glycoproteins associated with basement membranes and the extracellular matrix.
    J Cell Biol. 1982 Oct;95(1):197-204 PMID: 7142285
  15. Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis.
    Methods Enzymol. 1983;91:227-36 PMID: 6855576
  16. Nucleotide sequence of epidermal growth factor cDNA predicts a 128,000-molecular weight protein precursor.
    Nature. 1983 Jun 23-29;303(5919):722-5 PMID: 6304537
  17. Synthesis of laminin and entactin by F9 cells induced with retinoic acid and dibutyryl cyclic AMP.
    J Biol Chem. 1983 Jun 25;258(12):7729-37 PMID: 6305950
  18. Changes in the rate of laminin and entactin synthesis in F9 embryonal carcinoma cells treated with retinoic acid and cyclic amp.
    Dev Biol. 1983 Oct;99(2):510-6 PMID: 6194034
  19. Immunohistochemical localization of entactin and laminin in mouse embryos and fetuses.
    Dev Biol. 1983 Dec;100(2):496-505 PMID: 6653883
  20. Nidogen: a new, self-aggregating basement membrane protein.
    Eur J Biochem. 1983 Dec 15;137(3):455-65 PMID: 6420150
  21. The ultrastructural localization of two basement membrane components: entactin and laminin in rat tissues.
    J Histochem Cytochem. 1984 Mar;32(3):289-98 PMID: 6198358
  22. Distribution of entactin in the basement membrane of the rat mammary gland. Evidence for a non-epithelial origin.
    Exp Cell Res. 1984 May;152(1):240-54 PMID: 6538847
  23. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  24. The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA.
    Cell. 1984 Nov;39(1):27-38 PMID: 6091915
  25. Role of the conserved AAUAAA sequence: four AAUAAA point mutants prevent messenger RNA 3' end formation.
    Science. 1984 Nov 30;226(4678):1045-51 PMID: 6208611
  26. Matrix-cytoskeletal interactions in the developing eye.
    J Cell Biochem. 1985;27(2):143-56 PMID: 3886675
  27. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
    DNA. 1985 Apr;4(2):165-70 PMID: 3996185
  28. Laminin polymerization in vitro. Evidence for a two-step assembly with domain specificity.
    J Biol Chem. 1985 Jun 25;260(12):7636-44 PMID: 3997891
  29. Signal sequences. The limits of variation.
    J Mol Biol. 1985 Jul 5;184(1):99-105 PMID: 4032478
  30. Primary structure of bovine thyroglobulin deduced from the sequence of its 8,431-base complementary DNA.
    Nature. 1985 Aug 15-21;316(6029):647-51 PMID: 3855243
  31. Expression of nidogen and laminin in basement membranes during mouse embryogenesis and in teratocarcinoma cells.
    Dev Biol. 1985 Oct;111(2):372-82 PMID: 2995165
  32. Identification and interaction repertoire of large forms of the basement membrane protein nidogen.
    EMBO J. 1985 Oct;4(10):2513-8 PMID: 3932063
  33. Nature of sulphated macromolecules in mouse Reichert's membrane. Evidence for tyrosine O-sulphate in basement-membrane proteins.
    Biochem J. 1985 Nov 1;231(3):571-9 PMID: 4074325
  34. Homeotic gene products as growth factors.
    Cell. 1985 Dec;43(3 Pt 2):559-60 PMID: 3935324
  35. Basement membrane complexes with biological activity.
    Biochemistry. 1986 Jan 28;25(2):312-8 PMID: 2937447
  36. Purification and structural characterization of intact and fragmented nidogen obtained from a tumor basement membrane.
    Eur J Biochem. 1986 May 2;156(3):467-78 PMID: 3084254
  37. Structure, development, and molecular pathology of basement membranes.
    Int Rev Exp Pathol. 1986;29:1-112 PMID: 3536789
  38. The structure of human thrombospondin, an adhesive glycoprotein with multiple calcium-binding sites and homologies with several different proteins.
    J Cell Biol. 1986 Nov;103(5):1635-48 PMID: 2430973
  39. Control of laminin synthesis during differentiation of F9 embryonal carcinoma cells. A study using cDNA clones complementary to the mRNA species for the A, B1 and B2 subunits.
    Differentiation. 1986;32(3):260-6 PMID: 3025047
  40. Effect of basement membrane entactin on epidermal cell attachment and growth.
    J Invest Dermatol. 1987 Jan;88(1):55-9 PMID: 3794389
  41. Sequence of the cDNA encoding the laminin B1 chain reveals a multidomain protein containing cysteine-rich repeats.
    Proc Natl Acad Sci U S A. 1987 Feb;84(4):935-9 PMID: 3493487
  42. Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding.
    Cell. 1987 Mar 27;48(6):989-96 PMID: 2951015
  43. The receptor-binding sequence of urokinase. A biological function for the growth-factor module of proteases.
    J Biol Chem. 1987 Apr 5;262(10):4437-40 PMID: 3031025
  44. Carboxyl-terminal sequence of entactin deduced from a cDNA clone.
    Proc Natl Acad Sci U S A. 1987 Mar;84(6):1570-4 PMID: 3470744
  45. Acid-dependent ligand dissociation and recycling of LDL receptor mediated by growth factor homology region.
    Nature. 1987 Apr 23-29;326(6115):760-5 PMID: 3494949
  46. Laminin-nidogen complex. Extraction with chelating agents and structural characterization.
    Eur J Biochem. 1987 Jul 1;166(1):11-9 PMID: 3109910
  47. Two distinct cell-binding domains in laminin can independently promote nonneuronal cell adhesion and spreading.
    J Cell Biol. 1987 Jul;105(1):589-98 PMID: 3038930
  48. The cellular interactions of laminin fragments. Cell adhesion correlates with two fragment-specific high affinity binding sites.
    J Biol Chem. 1987 Aug 25;262(24):11532-8 PMID: 3114248
  49. Arginine-glycine-aspartic acid adhesion receptors.
    Methods Enzymol. 1987;144:475-89 PMID: 2442581
  50. New perspectives in cell adhesion: RGD and integrins.
    Science. 1987 Oct 23;238(4826):491-7 PMID: 2821619
  51. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
    Nucleic Acids Res. 1987 Oct 26;15(20):8125-48 PMID: 3313277
  52. Calcium binding domains and calcium-induced conformational transition of SPARC/BM-40/osteonectin, an extracellular glycoprotein expressed in mineralized and nonmineralized tissues.
    Biochemistry. 1987 Nov 3;26(22):6958-65 PMID: 3427055
  53. Primary structure of the mouse laminin B2 chain and comparison with laminin B1.
    Biochemistry. 1988 Jul 12;27(14):5198-204 PMID: 3167041
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.6.2749
Published
1988-12-00
Pages
2749-56
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115676
Subset
IM
Grants
NCI NIH HHS · CA21246 · United States
NIGMS NIH HHS · GM25690 · United States
Databases
GENBANK
X14194
External Links
PubMed source DOI: 10.1083/jcb.107.6.2749 PMC Full Text View journal details
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