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PMID: 3258664 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Endothelium-derived relaxing factor reduces platelet adhesion to bovine endothelial cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 8 ·1988-04-00 ·Pages 2800-4

Sneddon JM, Vane JR

Abstract

The adhesion of thrombin-stimulated human blood platelets to either the endothelial surface of intact bovine aorta or cultured bovine aortic endothelial cells was studied to determine the role of endothelium-derived relaxing factor in the regulation of platelet adhesion. Endothelial cells and platelets were pretreated with indomethacin to prevent the formation of prostaglandins. The adhesion of thrombin-stimulated platelets to endothelial cells was reduced by superoxide dismutase and bradykinin. The inhibitory effect of both drugs was abolished by hemoglobin and was absent in strips of bovine aorta where the endothelial cells had been removed by scraping. It is suggested that the effects of bradykinin are mediated by the release of endothelium-derived relaxing factor, which is protected from destruction by superoxide dismutase, and that endothelium-derived relaxing factor contributes to the nonadhesive properties of the vascular endothelium.

MeSH Terms
Adenine/metabolism Animals Biological Products/physiology Blood Platelets/physiology Bradykinin/pharmacology Cattle Endothelium, Vascular/cytology,physiology Humans In Vitro Techniques Nitric Oxide/pharmacology Platelet Adhesiveness Superoxide Dismutase/pharmacology Thrombin/pharmacology
Chemicals
Biological Products Nitric Oxide Superoxide Dismutase Thrombin Adenine Bradykinin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sneddon J M
William Harvey Research Institute, Saint Bartholomew's Hospital Medical College, London, United Kingdom.
Vane J R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.8.2800
Published
1988-04-00
Pages
2800-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280087
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.8.2800 PMC Full Text View journal details
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