Endothelial adenine uptake as an assay for cell- or complement-mediated cytotoxicity.

Immunology ·Vol. 32 ·No. 2 ·1977-02-00 ·Pages 221-6

DeBono DP, Macintyre DE, White DJ, Gordon JL

Abstract

[3H] Adenine uptake by monolayers of cultured pig aortic endothelium was characterized and used as the basis for a terminal adenine uptake cytotoxicity assay. This assay was used to demonstrate specific cytotoxicity against donor-type endothelium by serum and by peripheral blood lymphocytes from skin-graft allergized pigs, and to compare the time course of these two types of effector mechanism.

MeSH Terms

Adenine/metabolism Animals Antigen-Antibody Reactions Aorta/metabolism Cells, Cultured Complement System Proteins Cytotoxicity Tests, Immunologic/methods Endothelium/metabolism Lymphocytes/immunology Swine Time Factors

Chemicals

Complement System Proteins Adenine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

DeBono D P

Macintyre D E

White D J

Gordon J L

References (6)

6 references, click to expand

Preservation of tissue culture cells with liquid nitrogen.
Nature. 1962 Jun 9;194:993-4 PMID: 14477953
Stimulation of human lymphocytes by cultured allogeneic skin and endothelial cells in vitro.
Transplantation. 1975 Feb;19(2):191-4 PMID: 123372
The relationship of the histocompatibility locus in the pig to allograft survival.
Transplant Proc. 1973 Mar;5(1):317-20 PMID: 4266654
Histocompatibility phenotyping by the mixed lymphocyte reaction.
Tissue Antigens. 1973;3(4):340-7 PMID: 4272112
Endothelial cell stimulation of allogeneic lymphocytes.
Transplantation. 1972 Nov;14(5):652-4 PMID: 4264342
Interaction of pig lymphocytes with allogenic kidney cells in vitro.
Br J Exp Pathol. 1975 Aug;56(4):349-57 PMID: 1174460

Article Info

Journal

Immunology

Abbr.

Immunology

ISSN

0019-2805

Published

1977-02-00

Pages

221-6

Language

English

Region

England

NLM ID

0374672

PMCID

PMC1445219

Subset

External Links

PubMed source PMC Full Text View journal details

Analysis Services

Endothelial adenine uptake as an assay for cell- or complement-mediated cytotoxicity.

Contact

WeChat Official Account