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PMID: 3257575 Published · ppublish English Journal Article

Glucocorticoids selectively inhibit the transcription of the interleukin 1 beta gene and decrease the stability of interleukin 1 beta mRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 4 ·1988-02-00 ·Pages 1204-8

Lee SW, Tsou AP, Chan H, Thomas J, Petrie K, Eugui EM, Allison AC

Abstract

Transcription of the interleukin 1 beta (IL-1 beta) gene was studied by mRNA hybridization with a cDNA probe in the human promonocytic cell line U-937. Phorbol ester and lipopolysaccharide increased the steady-state level of IL-1 beta mRNA. Glucocorticoids markedly decreased IL-1 beta mRNA levels by two mechanisms. Transcription of the IL-1 gene was inhibited, as shown by in vitro transcription assays with nuclei isolated from glucocorticoid-treated cells. Moreover, kinetic analyses and pulse-labeling of mRNAs showed that glucocorticoids selectively decrease the stability of IL-1 beta mRNA, without affecting the stability of beta-actin and FOS mRNAs. Inhibition of the formation and effects IL-1 is a mechanism by which glucocorticoids can exert antiinflammatory and immunosuppressive effects.

MeSH Terms
Cycloheximide/pharmacology DNA/genetics Dactinomycin/pharmacology Depression, Chemical Dexamethasone/pharmacology Humans Hydrocortisone/pharmacology Interleukin-1/genetics Nucleic Acid Hybridization RNA, Messenger/metabolism Tetradecanoylphorbol Acetate/pharmacology Transcription, Genetic/drug effects Tumor Cells, Cultured
Chemicals
Interleukin-1 RNA, Messenger Dactinomycin Dexamethasone DNA Cycloheximide Tetradecanoylphorbol Acetate Hydrocortisone
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lee S W
Department of Immunology, Syntex Research, Palo Alto, CA 94303.
Tsou A P
Chan H
Thomas J
Petrie K
Eugui E M
Allison A C
References (41)
41 references, click to expand
  1. Establishment and characterization of a human histiocytic lymphoma cell line (U-937).
    Int J Cancer. 1976 May 15;17(5):565-77 PMID: 178611
  2. Interleukin-1 beta and interleukin-1 alpha stimulate the plasminogen activator activity and prostaglandin E2 levels of human synovial cells.
    Arthritis Rheum. 1987 May;30(5):562-6 PMID: 3109443
  3. Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct.
    J Biol Chem. 1979 Sep 25;254(18):9050-8 PMID: 479179
  4. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.
    Nucleic Acids Res. 1979 Nov 24;7(6):1541-52 PMID: 503860
  5. Selective reduction of proadrenocorticotropin/endorphin proteins and messenger ribonucleic acid activity in mouse pituitary tumor cells by glucocorticoids.
    Biochemistry. 1979 Oct 30;18(22):4907-15 PMID: 228704
  6. A phospholipase A2 inhibitory protein in rabbit neutrophils induced by glucocorticoids.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2533-6 PMID: 6930649
  7. Differential effects of glucocorticosteroids on the functions of subpopulations of helper T lymphocytes.
    Eur J Immunol. 1982 Jan;12(1):91-4 PMID: 6977454
  8. Prevention of the in vitro myelosuppressive effects of glucocorticosteroids by interleukin 1 (IL 1).
    J Immunol. 1982 Apr;128(4):1614-9 PMID: 6801116
  9. Glucocorticoids stimulate ribonucleic acid degradation in isolated rat thymic lymphocytes in vitro.
    Endocrinology. 1982 Jul;111(1):184-90 PMID: 6177526
  10. Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.
    J Biol Chem. 1982 Aug 10;257(15):8569-72 PMID: 7096324
  11. Modulation of interleukin 1 production by activated macrophages: in vitro action of hydrocortisone, colchicine, and cytochalasin B.
    Cell Immunol. 1982 May 15;69(2):235-47 PMID: 6809339
  12. Corticosteroid-mediated immunoregulation in man.
    Immunol Rev. 1982;65:133-55 PMID: 6214495
  13. Corticosteroids inhibit murine macrophage Ia expression and interleukin 1 production.
    J Immunol. 1982 Nov;129(5):1803-5 PMID: 6811653
  14. Selective protection of murine thymic helper T cells from glucocorticosteroid inhibition by macrophage-derived mediators.
    Cell Immunol. 1982 Oct;73(1):115-27 PMID: 6217899
  15. Prostaglandin biosynthesis in the human fetal adrenal gland: regulation by glucocorticosteroids.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7547-51 PMID: 6296839
  16. Gene expression in rat brain.
    Nucleic Acids Res. 1983 Aug 25;11(16):5497-520 PMID: 6193485
  17. Rapid suppression of alpha 1-fetoprotein gene transcription by dexamethasone in developing rat liver.
    Biochemistry. 1983 Aug 30;22(18):4296-302 PMID: 6194818
  18. Dexamethasone-mediated inhibition of human T cell growth factor and gamma-interferon messenger RNA.
    J Immunol. 1984 Jul;133(1):273-6 PMID: 6427338
  19. Glucocorticoid inhibition of lymphokine secretion by alloreactive T lymphocyte clones.
    J Immunol. 1984 Aug;133(2):784-91 PMID: 6203981
  20. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.
    Nature. 1984 Oct 4-10;311(5985):433-8 PMID: 6090941
  21. Pig catabolin is a form of interleukin 1. Cartilage and bone resorb, fibroblasts make prostaglandin and collagenase, and thymocyte proliferation is augmented in response to one protein.
    Biochem J. 1984 Dec 1;224(2):461-6 PMID: 6097218
  22. Reduction of serum Interleukin-1-like activity after treatment with dexamethasone.
    J Leukoc Biol. 1985 Feb;37(2):193-207 PMID: 3871468
  23. Glucocorticoid modulation of prostacyclin production in cultured bovine pulmonary endothelial cells.
    J Pharmacol Exp Ther. 1985 Jun;233(3):650-5 PMID: 3925123
  24. Prostacyclin synthesis induced in vascular cells by interleukin-1.
    Science. 1985 Jul 12;229(4709):174-6 PMID: 2409598
  25. Interleukin 1 acts on cultured human vascular endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes, and related leukocyte cell lines.
    J Clin Invest. 1985 Nov;76(5):2003-11 PMID: 3877078
  26. Steroid receptor regulated transcription of specific genes and gene networks.
    Annu Rev Genet. 1985;19:209-52 PMID: 3909942
  27. Human recombinant interleukin 1 stimulates collagenase and prostaglandin E2 production by human synovial cells.
    J Clin Invest. 1986 Feb;77(2):645-8 PMID: 3003163
  28. Interleukin 1 activates phospholipase A2 in rabbit chondrocytes: a possible signal for IL 1 action.
    J Immunol. 1986 Feb 15;136(4):1283-7 PMID: 3484767
  29. Actions of recombinant interleukin 1, interleukin 2, and interferon-gamma on bone resorption in vitro.
    J Immunol. 1986 Apr 1;136(7):2478-82 PMID: 3081643
  30. Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity.
    Nature. 1986 Mar 6-12;320(6057):77-81 PMID: 2936963
  31. Involvement of the 5'-leader sequence in coupling the stability of a human H3 histone mRNA with DNA replication.
    Proc Natl Acad Sci U S A. 1986 Feb;83(4):981-5 PMID: 3081892
  32. The cDNA sequence for the protein-tyrosine kinase substrate p36 (calpactin I heavy chain) reveals a multidomain protein with internal repeats.
    Cell. 1986 Jul 18;46(2):201-12 PMID: 3013423
  33. Glucocorticoids decrease the synthesis of type I procollagen mRNAs.
    Biochemistry. 1986 Jun 3;25(11):3202-9 PMID: 2425847
  34. A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
    Cell. 1986 Aug 29;46(5):659-67 PMID: 3488815
  35. Suppression of urokinase-type plasminogen activator mRNA levels in human fibrosarcoma cells and synovial fibroblasts by anti-inflammatory glucocorticoids.
    EMBO J. 1986 Sep;5(9):2217-22 PMID: 3780677
  36. Interleukin 1 releases histamine from human basophils and mast cells in vitro.
    J Immunol. 1987 Jan 1;138(1):271-5 PMID: 2431056
  37. Human monocyte or recombinant interleukin 1's are specific for the secretion of a metalloproteinase from chondrocytes.
    J Immunol. 1987 Jan 15;138(2):496-503 PMID: 3098847
  38. A superfamily of potentially oncogenic hormone receptors.
    Nature. 1986 Dec 18-31;324(6098):615-7 PMID: 3025739
  39. Inhibition of phospholipase A2 by "lipocortins" and calpactins. An effect of binding to substrate phospholipids.
    J Biol Chem. 1987 Feb 5;262(4):1698-705 PMID: 2948959
  40. Enhanced translation of chimaeric messenger RNAs containing a plant viral untranslated leader sequence.
    Nature. 1987 Feb 12-18;325(6105):622-5 PMID: 3492677
  41. Anti-inflammatory steroids induce biosynthesis of a phospholipase A2 inhibitor which prevents prostaglandin generation.
    Nature. 1979 Mar 29;278(5703):456-9 PMID: 450050
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.4.1204
Published
1988-02-00
Pages
1204-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279735
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.4.1204 PMC Full Text View journal details
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