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PMID: 3003163 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human recombinant interleukin 1 stimulates collagenase and prostaglandin E2 production by human synovial cells.

The Journal of clinical investigation ·Vol. 77 ·No. 2 ·1986-02-00 ·Pages 645-8

Dayer JM, de Rochemonteix B, Burrus B, Demczuk S, Dinarello CA

Abstract

The pathogenesis and progression of rheumatoid arthritis involves the production of biologically active lymphokines and monokines. Of these, interleukin 1 (IL-1) has been somewhat of a controversial molecule because it seems to evoke various biological responses in several different tissues. In these studies we demonstrate that three biological properties of human monocyte-derived IL-1 (T-lymphocyte activation and human synovial cell prostaglandin E2 and collagenase production) co-purify. The complementary DNA for the prominent pI 7 form of human IL-1 was expressed, purified, and tested. Any controversy now appears resolved since homogeneous recombinant human IL-1 stimulates prostaglandin E2 and collagenase from human synovial cells as well as activates T cells in vitro.

MeSH Terms
Animals Arthritis, Rheumatoid/metabolism Cells, Cultured Chromatography DNA, Recombinant Dinoprostone Escherichia coli/genetics Humans Interleukin-1/genetics,physiology Lymphocyte Activation Mice Mice, Inbred C3H Microbial Collagenase/biosynthesis Monocytes/metabolism Prostaglandins E/biosynthesis Recombinant Proteins Synovial Membrane/metabolism
Chemicals
DNA, Recombinant Interleukin-1 Prostaglandins E Recombinant Proteins Microbial Collagenase Dinoprostone
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dayer J M
de Rochemonteix B
Burrus B
Demczuk S
Dinarello C A
References (12)
12 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI112350
Published
1986-02-00
Pages
645-8
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC423407
Subset
IM
Grants
NIAID NIH HHS · AI 15614 · United States
External Links
PubMed source DOI: 10.1172/JCI112350 PMC Full Text View journal details
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