Home LiteratureArticle Details
PMID: 325517 Published · ppublish English Journal Article

Requirement of chain initiation factor 3 and ribosomal protein S1 in translation of synthetic and natural messenger RNA.

Nucleic acids research ·Vol. 4 ·No. 1 ·1977-01-00 ·Pages 17-29

Sobura JE, Chowdhury MR, Hawley DA, Wahba AJ

Abstract

Amino acid incorporation directed by poly(A), poly(U) or R17 RNA has been examined in S1-depleted protein synthesizing systems. We observe that the translation of either synthetic or natural messenger RNA is strictly dependent on the presence of chain initiation factor 3 and ribosomal protein S1. With poly(A) or poly(U) both IF-3 and S1 stimulate amino acid incorporation at least 25-fold, and with R17 RNA the stimulation is approximately 15-fold. More than one copy of S1 per ribosome decreases amino acid incorporation directed by poly(U) or R17 RNA. Initiation complex formation with R17 RNA is also stimulated optimally by the addition of one copy of S1 per ribosome. The function of IF-3 and S1 in protein synthesis is considered.

MeSH Terms
Bacterial Proteins/pharmacology Coliphages Escherichia coli Peptide Initiation Factors Poly A/metabolism Poly U/metabolism Polynucleotides/metabolism Protein Biosynthesis RNA, Messenger/metabolism RNA, Viral/metabolism Ribosomal Proteins/pharmacology Ribosomes/metabolism
Chemicals
Bacterial Proteins Peptide Initiation Factors Polynucleotides RNA, Messenger RNA, Viral Ribosomal Proteins Poly A Poly U
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sobura J E
Chowdhury M R
Hawley D A
Wahba A J
References (36)
36 references, click to expand
  1. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  2. Subunit I of G beta replicase and 30 S ribosomal protein S1 of Escherichia coli. Evidence for the identity of the two proteins.
    J Biol Chem. 1974 May 25;249(10):3314-6 PMID: 4208476
  3. Physical and functional homology between ribosomal protein S1 and interference factor i.
    Eur J Biochem. 1974 Jun 1;45(1):109-17 PMID: 4213953
  4. Interaction of Escherichia coli 30S ribosomal subunits with MS2 phage RNA in the absence of initiation factors.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3611-5 PMID: 4610580
  5. Two forms of the 30 S ribosomal subunit of Escherichia coli.
    J Biol Chem. 1974 Dec 10;249(23):7673-8 PMID: 4612039
  6. The mechanism of action of initiation factor 3 in protein synthesis. I. Studies on ribosomes crosslinked with dimethylsuberimidate.
    Biochem Biophys Res Commun. 1974 Nov 6;61(1):329-37 PMID: 4613353
  7. Replacement of ribosomal protein S1 by interference factor ialpha in ribosomal binding of phage Ms2 RNA.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4708-12 PMID: 4612526
  8. The mechanism of action of initiation factor 3 in protein synthesis. II. Association of the 30S ribosomal protein S12 with IF-3.
    Biochem Biophys Res Commun. 1974 Nov 27;61(2):544-50 PMID: 4616691
  9. Chain initiation factors from Escherichia coli.
    Methods Enzymol. 1974;30:3-18 PMID: 4604199
  10. Polyuridylic acid binding and translating by Escherichia coli ribosomes: stimulation by protein I, inhibition by aurintricarboxylic acid.
    Biochim Biophys Acta. 1972 Oct 27;281(3):381-92 PMID: 4565240
  11. Chain initiation factor 2. Purification and properties of two species from Escherichia coli MRE 600.
    J Biol Chem. 1973 Feb 10;248(3):1084-90 PMID: 4567787
  12. Functional heterogeneity of the 30 S ribosomal subunit of Escherichia coli. 3. Requirement of protein S1 for translation.
    J Mol Biol. 1974 Mar 25;84(1):185-95 PMID: 4598364
  13. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  14. Cistron specificity of 30S ribosomes heterologously reconstituted with components from Escherichia coli and Bacillus stearothermophilus.
    Biochemistry. 1974 May 7;13(10):2123-9 PMID: 4597073
  15. Role of 16S ribosomal ribonucleic acid and the 30S ribosomal protein S12 in the initiation of natural messenger ribonucleic acid translation.
    Biochemistry. 1974 May 7;13(10):2115-22 PMID: 4597072
  16. Inhibition of synthetic and natural messenger translation. II. Specificity and mechanism of action of a protein isolated from Escherichia coli MRE 600 ribosomes.
    J Biol Chem. 1974 Jun 25;249(12):3808-13 PMID: 4600241
  17. Inhibition of synthetic and natural messenger translation. I. Purification and properties of a protein isolated from Escherichia coli MRE 600 ribosomes.
    J Biol Chem. 1974 Jun 25;249(12):3803-7 PMID: 4601013
  18. Purification and properties of chain initiation factor 3 from T4-infected and uninfected Escherichia coli MRE 600. Stimulation of translation of synthetic and natural messengers.
    J Biol Chem. 1974 Jun 25;249(12):3797-802 PMID: 4601012
  19. A protein-nucleic acid crosslink in 30S ribosomes.
    Biochem Biophys Res Commun. 1973 Apr 16;51(4):932-8 PMID: 4703562
  20. Translation of the genetic message. VI. The role of ribosomal subunits in binding of formylmethionyl-tRNA and its reaction with puromycin.
    Proc Natl Acad Sci U S A. 1967 Oct;58(4):1652-4 PMID: 4867669
  21. Translation of the genetic message. VII. Role of initiation factors in formation of the chain initiation complex with Escherichia coli ribosomes.
    Arch Biochem Biophys. 1968 May;125(2):542-7 PMID: 4872321
  22. Isolation of bacterial and phage proteins by homopolymer RNA-cellulose chromatography.
    J Biol Chem. 1975 Aug 10;250(15):6160-7 PMID: 807580
  23. Structure of the poly(G) polymerase component of the bacteriophage f2 replicase.
    Proc Natl Acad Sci U S A. 1971 Aug;68(8):1838-43 PMID: 5288771
  24. Translational repression of a viral messenger RNA by a host protein.
    J Biol Chem. 1975 Aug 10;250(15):5749-55 PMID: 1097443
  25. Photoaffinity reaction between polyuridylic acid and protein S1 on the Escherichia coli ribosome.
    FEBS Lett. 1975 Apr 1;52(2):281-3 PMID: 1093872
  26. Ribosomal protein S1 and polypeptide chain initiation in bacteria.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2325-9 PMID: 1094462
  27. Function of Escherichia coli ribosomal protein S1 in translation of natural and synthetic messenger RNA.
    J Mol Biol. 1975 Apr 15;93(3):351-66 PMID: 1095761
  28. Binding of ribosomal protein S1 of Escherichia coli to the 3' end of 16S rRNA.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):2940-4 PMID: 1103129
  29. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734-8 PMID: 1107998
  30. New aspects of the IF3-ribosome interaction.
    FEBS Lett. 1976 Feb 15;62(2):111-4 PMID: 767141
  31. Destabilization of the secondary structure of RNA by ribosomal protein S1 from Escherichia coli.
    Biochem Biophys Res Commun. 1976 Jun 7;70(3):957-64 PMID: 779790
  32. Alteration of polynucleotide secondary structure by ribosomal protein S1.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1824-8 PMID: 778845
  33. Site-specific interaction of Qbeta host factor and ribosomal protein S1 with Qbeta and R17 bacteriophage RNAs.
    J Biol Chem. 1976 Apr 10;251(7):1902-12 PMID: 773930
  34. 30S ribosomal proteins associated with the 3'-terminus of 16S RNA.
    FEBS Lett. 1975 Oct 15;58(1):281-4 PMID: 1225593
  35. Guanosine 5'-triphosphatase activity of initiation factor f2.
    Proc Natl Acad Sci U S A. 1968 Nov;61(3):1066-70 PMID: 5246542
  36. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.1.17
Published
1977-01-00
Pages
17-29
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342406
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.1.17 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2