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PMID: 324909 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Use of a free viral immunofluorescence assay to detect human reovirus-like agent in human stools.

Infection and immunity ·Vol. 16 ·No. 2 ·1977-05-00 ·Pages 467-70

Yolken RH, Wyatt RG, Kalica AR, Kim HW, Brandt CD, Parrott RH, Kapikian AZ, Chanock RM

Abstract

Human reovirus-like agent (HRVLA) is a major cause of gastroenteritis in infants and young children in many parts of the world. Detection of HRVLA in stools is impractical with the techniques currently available. We describe a rapid immunofluorescence assay for detection of HRVLA in stools. Results with this assay agreed well with results obtained from examination of stool specimens by electron microscopy.

MeSH Terms

Animals Antigen-Antibody Complex Binding, Competitive Cattle Feces/microbiology Fluorescent Antibody Technique Guinea Pigs Humans Reoviridae/isolation & purification,ultrastructure Swine

Chemicals

Antigen-Antibody Complex

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Yolken R H

Wyatt R G

Kalica A R

Kim H W

Brandt C D

Parrott R H

Kapikian A Z

Chanock R M

References (14)

14 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.16.2.467-470.1977

Published

1977-05-00

Pages

467-70

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC420974

Subset

External Links

PubMed source DOI: 10.1128/iai.16.2.467-470.1977 PMC Full Text View journal details

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Use of a free viral immunofluorescence assay to detect human reovirus-like agent in human stools.

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