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PMID: 3241623 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Identification and nucleotide sequence of the class E tet regulatory elements and operator and inducer binding of the encoded purified Tet repressor.

Molecular & general genetics : MGG ·Vol. 215 ·No. 1 ·1988-12-00 ·Pages 76-80

Tovar K, Ernst A, Hillen W

Abstract

The regulatory region and repressor (tetRE) gene from the class E tetracycline resistance determinant previously isolated from Enterobacteriaceae have been identified and completely sequenced. The regulatory region is located between the resistance gene and the tetR gene which have opposite polarity. The tetR gene encodes a protein consisting of 211 amino acids with a calculated molecular weight of 23.6 kDa. Cloning of the tetR gene under transcriptional control of the lambda PL promoter leads to overexpression of a polypeptide with an apparent molecular weight of 26 kDa. The purified protein binds sequence specifically to DNA fragments containing putative tet operators. This property is lost in the presence of tetracycline. The relationship of the tetRE sequence to four known tetracycline resistance determinants is discussed.

MeSH Terms

Amino Acid Sequence Base Sequence Binding Sites DNA, Bacterial/genetics Enterobacteriaceae/genetics,metabolism Genes, Bacterial Genes, Regulator Molecular Sequence Data Operon Promoter Regions, Genetic Repressor Proteins/genetics,metabolism Tetracycline Resistance/genetics

Chemicals

DNA, Bacterial Repressor Proteins tetracycline resistance-encoding transposon repressor protein

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Tovar K

Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Federal Republic of Germany.

Ernst A

Hillen W

References (22)

22 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00331306

Published

1988-12-00

Pages

76-80

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00331306

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Identification and nucleotide sequence of the class E tet regulatory elements and operator and inducer binding of the encoded purified Tet repressor.

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