Home LiteratureArticle Details
PMID: 3237211 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Selection and characterization of ura5 mutants of Histoplasma capsulatum.

Molecular & general genetics : MGG ·Vol. 214 ·No. 2 ·1988-10-00 ·Pages 348-52

Worsham PL, Goldman WE

Abstract

The combined use of non-aggregating Histoplasma capsulatum strains and a defined medium which allows quantitative plating of the yeast phase has allowed us to select 5-fluoroorotic acid (5-FOA)-resistant mutants of this dimorphic fungus. Approximately two-thirds of the 5-FOA-resistant strains were auxotrophic for uracil; all were deficient in orotidine-5'-monophosphate pyrophosphorylase (OMPpase) activity. One class of OMPpase mutant (alpha), which retained a low level of OMPpase activity, was auxotrophic in the yeast phase (37 degrees C) but grew slowly in the mycelial phase (25 degrees C) without exogenous uracil. This phenotype was not due to a temperature-sensitive OMPpase activity. Both wild-type and alpha mutants had a higher OMPpase activity in the mycelial phase than the yeast phase; this increased activity may be sufficient to allow mycelial growth of alpha mutants.

MeSH Terms
Histoplasma/genetics,growth & development,radiation effects Mutation Orotate Phosphoribosyltransferase/genetics Orotidine-5'-Phosphate Decarboxylase/genetics Ultraviolet Rays Uracil/metabolism
Chemicals
Uracil Orotate Phosphoribosyltransferase Orotidine-5'-Phosphate Decarboxylase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Worsham P L
Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, MO 63110.
Goldman W E
References (18)
18 references, click to expand
  1. Quantitative plating of Histoplasma capsulatum without addition of conditioned medium or siderophores.
    J Med Vet Mycol. 1988 Jun;26(3):137-43 PMID: 3171821
  2. Regulation of orotidylic acid pyrophosphorylase in Saccharomyces cerevisiae.
    J Bacteriol. 1972 Jan;109(1):196-202 PMID: 4550660
  3. Classification of Histoplasma capsulatum isolates by restriction fragment polymorphisms.
    J Bacteriol. 1986 Mar;165(3):813-8 PMID: 3005239
  4. Hydroxamic acid from Histoplasma capsulatum that displays growth factor activity.
    Appl Environ Microbiol. 1981 Sep;42(3):560-3 PMID: 7294789
  5. Positive screening and transformation of ura5 mutants in the fungus Podospora anserina: characterization of the transformants.
    Curr Genet. 1986;10(11):811-7 PMID: 3447739
  6. Orotate phosphoribosyltransferase: orotidylate decarboxylase (Ehrlich ascites cell).
    Methods Enzymol. 1978;51:155-67 PMID: 692383
  7. Histoplasma capsulatum fails to trigger release of superoxide from macrophages.
    Infect Immun. 1987 Jan;55(1):29-34 PMID: 3025100
  8. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli.
    J Mol Biol. 1962 Dec;5:618-34 PMID: 13970180
  9. Isolation and characterization of spontaneous avirulent variants of Histoplasma capsulatum.
    Infect Immun. 1987 Mar;55(3):528-33 PMID: 3818083
  10. Studies on Emmonsiella capsulata. I. Heterothallism and development of the ascocarp.
    Mycologia. 1973 Jan-Feb;65(1):109-21 PMID: 4686214
  11. The ura5 gene of the filamentous fungus Podospora anserina: nucleotide sequence and expression in transformed strains.
    Gene. 1987;53(2-3):201-9 PMID: 3609748
  12. Cysteine-independent and cysteine-requiring yeast-strains of Histoplasma capsulatum.
    Mycopathologia. 1982 Feb 19;77(2):69-73 PMID: 7070482
  13. Genetic and physiological aspects of resistance to 5-fluoropyrimidines in Saccharomyces cerevisiae.
    J Bacteriol. 1970 Jun;102(3):607-15 PMID: 5429721
  14. Cysteine transport and sulfite reductase activity in a germination-defective mutant of Histoplasma capsulatum.
    J Bacteriol. 1980 Jan;141(1):417-21 PMID: 7354005
  15. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.
    Mol Gen Genet. 1984;197(2):345-6 PMID: 6394957
  16. Cloning gene ura5 for the orotidylic acid pyrophosphorylase of the filamentous fungus Podospora anserina: transformation of protoplasts.
    Gene. 1984 Dec;32(3):487-92 PMID: 6530144
  17. Selenocystine-resistant mutants of Histoplasma capsulatum.
    Mycopathologia. 1981 Mar 13;73(3):177-81 PMID: 7219517
  18. Emmonsiella capsulata: perfect state of Histoplasma capsulatum.
    Science. 1972 Jul 28;177(4046):368-9 PMID: 5035491
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00337734
Published
1988-10-00
Pages
348-52
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIAID NIH HHS · AI07015 · United States
External Links
PubMed source DOI: 10.1007/BF00337734
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2