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PMID: 322130 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Virion-associated RNA polymerase required for bacteriophage N4 development.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 2 ·1977-02-00 ·Pages 520-3

Falco SC, Laan KV, Rothman-Denes LB

Abstract

Escherichia coli phage N4 transcription is resistant to rifampicin, an inhibitor of the host RNA polymerase, even when the drug is added prior to infection. A rifampicin-resistant RNA polymerase has been detected in disrupted N4 virions. This enzyme shows a requirement for the four ribonucleoside 5'-triphosphates and exogenous denatured DNA. With denatured N4 DNA, the preferred template, transcription is asymmetric. The virion RNA polymerase apparently is necessary for phage development because a conditional lethal N4 mutant shows temperature-sensitive RNA synthesis in vivo as well as a temperature-sensitive RNA polymerase in disrupted virions.

MeSH Terms
Binding, Competitive Chloramphenicol/pharmacology Coliphages/drug effects,enzymology,physiology DNA-Directed RNA Polymerases/metabolism Escherichia coli/drug effects,enzymology Kinetics Nucleic Acid Hybridization Templates, Genetic Transcription, Genetic/drug effects Virus Replication
Chemicals
Chloramphenicol DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Falco S C
Laan K V
Rothman-Denes L B
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.2.520
Published
1977-02-00
Pages
520-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392321
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.2.520 PMC Full Text View journal details
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