Home LiteratureArticle Details
PMID: 3196167 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Fine structure of the vaccinia virus gene encoding the precursor of the major core protein 4 a.

Archives of virology ·Vol. 102 ·No. 1-2 ·1988-00-00 ·Pages 19-27

Van Meir E, Wittek R

Abstract

A 6008 base pair fragment of the vaccinia virus DNA containing the gene for the precursor of the major core protein 4 a, which has been designated P4 a, was sequenced. A long open reading frame (ORF) encoding a protein of molecular weight 102,157 started close to the position where the P4 a mRNA had been mapped. Analysis of the mRNA by S1 nuclease mapping and primer extension indicated that the 5' end defined by the former method is not the true 5' end. This suggests that the P4 a coding region is preceded by leader sequences that are not derived from the immediate vicinity of the gene, similar to what has been reported for another late vaccinia virus mRNA. The sequenced DNA contained several further ORFs on the same, or opposite DNA strand, providing further evidence for the close spacing of protein-coding sequences in the viral genome.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Viral/genetics Genes, Viral Molecular Sequence Data Protein Precursors/genetics RNA, Messenger/genetics Restriction Mapping Vaccinia virus/genetics Viral Core Proteins/genetics
Chemicals
DNA, Viral Protein Precursors RNA, Messenger Viral Core Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Van Meir E
Institut de Biologie animale, Université de Lausanne, Switzerland.
Wittek R
References (20)
20 references, click to expand
  1. One hundred base pairs of 5' flanking sequence of a vaccinia virus late gene are sufficient to temporally regulate late transcription.
    Proc Natl Acad Sci U S A. 1985 Apr;82(7):2096-100 PMID: 3856886
  2. DNA sequence comparison between two tissue-specific variants of the autonomous parvovirus, minute virus of mice.
    Nucleic Acids Res. 1985 May 24;13(10):3617-33 PMID: 3855242
  3. Transcriptional and translational mapping and nucleotide sequence analysis of a vaccinia virus gene encoding the precursor of the major core polypeptide 4b.
    J Virol. 1985 Dec;56(3):830-8 PMID: 2999438
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  6. Letter: Protein cleavage and poxvirus morphogenesis: tryptic peptide analysis of core precursors accumulated by blocking assembly with rifampicin.
    J Mol Biol. 1973 Dec 5;81(2):267-9 PMID: 4777308
  7. Formation of a vaccinia virus structural polypeptide from a higher molecular weight precursor: inhibition by rifampicin.
    Proc Natl Acad Sci U S A. 1970 Jul;66(3):677-84 PMID: 5269234
  8. Studies on the nature and location of the capsid polypeptides of vaccinia virions.
    Virology. 1972 Nov;50(2):579-92 PMID: 4117937
  9. Vaccinia virus produces late mRNAs by discontinuous synthesis.
    Cell. 1987 Jul 17;50(2):153-62 PMID: 3036368
  10. Nucleotide sequence and genetic map of the 16-kb vaccinia virus HindIII D fragment.
    Virology. 1986 Aug;153(1):96-112 PMID: 3739227
  11. Regulation of expression and nucleotide sequence of a late vaccinia virus gene.
    J Virol. 1984 Sep;51(3):662-9 PMID: 6088791
  12. A tandemly-oriented late gene cluster within the vaccinia virus genome.
    Nucleic Acids Res. 1986 Apr 11;14(7):3003-16 PMID: 3008103
  13. Nucleotide sequence of a cluster of early and late genes in a conserved segment of the vaccinia virus genome.
    Nucleic Acids Res. 1985 Feb 11;13(3):985-98 PMID: 2987815
  14. Kilo-sequencing: an ordered strategy for rapid DNA sequence data acquisition.
    Nucleic Acids Res. 1983 Jan 25;11(2):349-68 PMID: 6298723
  15. Mapping of a gene coding for a major late structural polypeptide on the vaccinia virus genome.
    J Virol. 1984 Feb;49(2):371-8 PMID: 6319738
  16. Conserved TAAATG sequence at the transcriptional and translational initiation sites of vaccinia virus late genes deduced by structural and functional analysis of the HindIII H genome fragment.
    J Virol. 1986 Nov;60(2):436-49 PMID: 3021979
  17. Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5' poly(A) leader.
    Cell. 1987 Jul 17;50(2):163-9 PMID: 3594569
  18. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  19. Biogenesis of vaccinia: evidence for more than 100 polypeptides in the virion.
    Virology. 1979 Jun;95(2):385-94 PMID: 462799
  20. Mapping of the genes coding for the two major vaccinia virus core polypeptides.
    Nucleic Acids Res. 1984 Jun 25;12(12):4835-48 PMID: 6330686
Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01315559
Published
1988-00-00
Pages
19-27
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01315559 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2