Home LiteratureArticle Details
PMID: 3008103 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A tandemly-oriented late gene cluster within the vaccinia virus genome.

Nucleic acids research ·Vol. 14 ·No. 7 ·1986-04-11 ·Pages 3003-16

Weinrich SL, Hruby DE

Abstract

The nucleotide sequence of a 5.1 kilobase-pair fragment from the central portion of the vaccinia virus genome has been determined. Within this region, five complete and two incomplete open reading frames (orfs) are tightly-clustered, tandemly-oriented, and read in the leftward direction. Late mRNA start sites for the five complete orfs and one incomplete orf were determined by S1 nuclease mapping. The two leftmost complete orfs correlated with late polypeptides of 65,000 and 32,000 molecular weight previously mapped to this region. When compared with each other and with sequences present in protein data banks, the five complete orfs showed no significant homology matches amongst themselves or any previously reported sequence. The six putative promoters were aligned with three previously sequenced late gene promoters. While all of the nine are A-T rich, the only apparent consensus sequence is TAA immediately preceeding the initiator ATG. Identification of this tandemly-oriented late gene cluster suggests local organization of the viral genome.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Restriction Enzymes/metabolism Deoxyribonuclease HindIII Deoxyribonucleases, Type II Site-Specific Endonucleases/metabolism Molecular Weight RNA, Messenger/analysis Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic Vaccinia virus/genetics
Chemicals
RNA, Messenger Endonucleases DNA Restriction Enzymes Deoxyribonuclease HindIII Deoxyribonucleases, Type II Site-Specific GTCGAC-specific type II deoxyribonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Weinrich S L
Hruby D E
References (41)
41 references, click to expand
  1. Intermolecular duplexes formed from polyadenylylated vaccinia virus RNA.
    J Virol. 1979 Apr;30(1):365-74 PMID: 480457
  2. Neomycin resistance as a dominant selectable marker for selection and isolation of vaccinia virus recombinants.
    Mol Cell Biol. 1985 Aug;5(8):1918-24 PMID: 3018537
  3. Conservation and variation in Orthopoxvirus genome structure.
    J Gen Virol. 1979 Dec;45(3):683-701 PMID: 232137
  4. Expression of the vaccinia virus genome: analysis and mapping of mRNAs encoded within the inverted terminal repetition.
    Cell. 1980 Sep;21(2):487-93 PMID: 7407921
  5. A map of the late proteins of vaccinia virus.
    Virology. 1980 Aug;105(1):261-4 PMID: 6251610
  6. Cell-free translation of early and late mRNAs selected by hybridization to cloned DNA fragments derived from the left 14 million to 72 million daltons of the vaccinia virus genome.
    Virology. 1981 Jul 15;112(1):306-17 PMID: 6264687
  7. Distinctive nucleotide sequences adjacent to multiple initiation and termination sites of an early vaccinia virus gene.
    Cell. 1981 Sep;25(3):805-13 PMID: 7285118
  8. Extension of the transcriptional and translational map of the left end of the vaccinia virus genome to 21 kilobase pairs.
    J Virol. 1981 Sep;39(3):733-45 PMID: 6270348
  9. Organization and expression of the poxvirus genome.
    Experientia. 1982 Mar 15;38(3):285-97 PMID: 6281055
  10. Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus.
    Proc Natl Acad Sci U S A. 1982 Aug;79(16):4927-31 PMID: 6289324
  11. Complete nucleotide sequences of two adjacent early vaccinia virus genes located within the inverted terminal repetition.
    J Virol. 1982 Nov;44(2):637-46 PMID: 7143577
  12. Vaccinia virus: a selectable eukaryotic cloning and expression vector.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7415-9 PMID: 6296831
  13. Hybridization and sedimentation studies on "early" and "late" vaccinia messenger RNA.
    J Mol Biol. 1967 Aug 14;27(3):395-419 PMID: 6049681
  14. RNA polymerase activity in purified infectious vaccinia virus.
    Proc Natl Acad Sci U S A. 1967 Dec;58(6):2280-7 PMID: 5242206
  15. Sequential protein synthesis following vaccinia virus infection.
    J Virol. 1968 Oct;2(10):1016-27 PMID: 4235537
  16. Mechanism of synthesis of vaccinia virus double-stranded ribonucleic acid in vivo and in vitro.
    J Virol. 1971 Jan;7(1):71-6 PMID: 5543434
  17. Letter: Protein cleavage and poxvirus morphogenesis: tryptic peptide analysis of core precursors accumulated by blocking assembly with rifampicin.
    J Mol Biol. 1973 Dec 5;81(2):267-9 PMID: 4777308
  18. Vaccinia virus polypeptide synthesis: sequential appearance and stability of pre- and post-replicative polypeptides.
    J Gen Virol. 1974 Dec;25(3):433-44 PMID: 4216621
  19. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  20. Transcriptional complexity of vaccinia virus in vivo and in vitro.
    J Virol. 1977 Sep;23(3):608-15 PMID: 894791
  21. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  22. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
    J Mol Biol. 1978 Mar 25;120(1):97-120 PMID: 642007
  23. Vaccinia virus transcription: hybridization of mRNA to restriction fragments of vaccinia DNA.
    Virology. 1978 May 1;86(1):102-14 PMID: 664221
  24. Sequence complexity and relative abundance of vaccinia virus mRNA's synthesized in vivo and in vitro.
    J Virol. 1978 Jun;26(3):554-69 PMID: 671583
  25. Nucleotide sequence of the vaccinia virus thymidine kinase gene and the nature of spontaneous frameshift mutations.
    J Virol. 1983 May;46(2):530-7 PMID: 6842679
  26. Fine structure analysis and nucleotide sequence of the vaccinia virus thymidine kinase gene.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3411-5 PMID: 6304709
  27. Mapping of a gene coding for a major late structural polypeptide on the vaccinia virus genome.
    J Virol. 1984 Feb;49(2):371-8 PMID: 6319738
  28. Organization of six early transcripts synthesized from a vaccinia virus EcoRI DNA fragment.
    J Virol. 1984 Feb;49(2):497-509 PMID: 6319749
  29. Arrangement of late RNAs transcribed from a 7.1-kilobase EcoRI vaccinia virus DNA fragment.
    J Virol. 1984 Feb;49(2):510-20 PMID: 6319750
  30. A comprehensive sequence analysis program for the IBM personal computer.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):581-99 PMID: 6546431
  31. General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes.
    J Virol. 1984 Mar;49(3):857-64 PMID: 6321770
  32. Mapping of the genes coding for the two major vaccinia virus core polypeptides.
    Nucleic Acids Res. 1984 Jun 25;12(12):4835-48 PMID: 6330686
  33. Regulation of expression and nucleotide sequence of a late vaccinia virus gene.
    J Virol. 1984 Sep;51(3):662-9 PMID: 6088791
  34. One hundred base pairs of 5' flanking sequence of a vaccinia virus late gene are sufficient to temporally regulate late transcription.
    Proc Natl Acad Sci U S A. 1985 Apr;82(7):2096-100 PMID: 3856886
  35. Nucleotide sequence of a cluster of early and late genes in a conserved segment of the vaccinia virus genome.
    Nucleic Acids Res. 1985 Feb 11;13(3):985-98 PMID: 2987815
  36. Isolation of cis-acting vaccinia virus DNA fragments promoting the expression of herpes simplex virus thymidine kinase by recombinant viruses.
    J Virol. 1985 Jul;55(1):163-72 PMID: 2989553
  37. Transcriptional and translational analysis of the vaccinia virus late gene L65.
    J Virol. 1985 Aug;55(2):450-7 PMID: 2991568
  38. Recombinant vaccinia virus: immunization against multiple pathogens.
    Science. 1985 Sep 6;229(4717):981-4 PMID: 2992092
  39. Transcriptional and translational mapping and nucleotide sequence analysis of a vaccinia virus gene encoding the precursor of the major core polypeptide 4b.
    J Virol. 1985 Dec;56(3):830-8 PMID: 2999438
  40. Physical mapping and DNA sequence analysis of the rifampicin resistance locus in vaccinia virus.
    Virology. 1985 Dec;147(2):394-404 PMID: 3000072
  41. Translation of specific vaccinia virus RNAs purified as RNA-DNA hybrids on potassium iodide gradients.
    Nucleic Acids Res. 1979 Aug 10;6(11):3599-612 PMID: 493115
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.7.3003
Published
1986-04-11
Pages
3003-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339717
Subset
IM
Grants
NIAID NIH HHS · AI-00666 · United States
NIAID NIH HHS · AI-20563 · United States
Databases
GENBANK
X03729
External Links
PubMed source DOI: 10.1093/nar/14.7.3003 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2