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PMID: 3194199 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Resolution of synthetic Holliday structures by an extract of human cells.

Nucleic acids research ·Vol. 16 ·No. 21 ·1988-11-11 ·Pages 10249-66

Waldman AS, Liskay RM

Abstract

Virtually all models for recombination between homologous DNA sequences invoke a branched intermediate known as a Holliday structure. The terminal steps of recombination are postulated to involve a specific cleavage through the four-way junction of a Holliday structure, in a process known as resolution. We have constructed a synthetic Holliday structure in which the position of the junction of the DNA duplexes can branch migrate through approximately 185 bp. Using this structure, we have found that a component of a cytoplasmic extract of Hela cells is capable of cleaving the central junction of the substrate in a manner consistent with resolution. The activity requires a divalent cation but does not require an exogenous energy source. This is the first reported resolution activity from a mammalian source.

MeSH Terms
Cell Nucleus/metabolism Cytosol/metabolism DNA/genetics HeLa Cells/metabolism Humans Models, Genetic Oligodeoxyribonucleotides/metabolism Recombination, Genetic
Chemicals
Oligodeoxyribonucleotides DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Waldman A S
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT 06510.
Liskay R M
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.21.10249
Published
1988-11-11
Pages
10249-66
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338850
Subset
IM
Grants
NIGMS NIH HHS · GM32741 · United States
External Links
PubMed source DOI: 10.1093/nar/16.21.10249 PMC Full Text View journal details
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