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PMID: 319250 Published · ppublish English Journal Article

Streptomycin-resistant Escherichia coli mutant temperature sensitive for the production of Qbeta-infective particles.

Journal of virology ·Vol. 21 ·No. 1 ·1977-01-00 ·Pages 1-6

Engelberg-Kulka H, Dekel L, Israeli-Reches M

Abstract

A streptomycin-resistant Escherichia coli mutant has been isolated that is temperature sensitive for Qbeta phage, but not for the group I RNA phages f2, MS2, and R17. The growth of Qbeta in the mutant at the nonpermissive temperature (42 degrees C) results in the release of a near-normal burst of noninfectious particles that cosediment with Qbeta in a sucrose gradient. It is assumed that the mutant is defective at elevated temperatures in the suppression of nonsense codons, thereby producing Qbeta-like particles which are noninfectious because of the lack of the read-through protein A1.

MeSH Terms
Coliphages/growth & development,metabolism Escherichia coli/drug effects Lysogeny Mutation RNA, Viral/biosynthesis Streptomycin/pharmacology Temperature Virus Replication
Chemicals
RNA, Viral Streptomycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Engelberg-Kulka H
Dekel L
Israeli-Reches M
References (33)
33 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.21.1.1-6.1977
Published
1977-01-00
Pages
1-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353784
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.21.1.1-6.1977 PMC Full Text View journal details
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