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PMID: 3186746 Published · ppublish English Journal Article

Construction of a genetic map of human chromosome 17 by use of chromosome-mediated gene transfer.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 22 ·1988-11-00 ·Pages 8563-7

Xu WM, Gorman PA, Rider SH, Hedge PJ, Moore G, Prichard C, Sheer D, Solomon E

Abstract

We used somatic-cell hybrids, containing as their only human genetic contribution part or all of chromosome 17, as donors for chromosome-mediated gene transfer. A total of 54 independent transfectant clones were isolated and analyzed by use of probes or isoenzymes for greater than 20 loci located on chromosome 17. By combining the data from this chromosome-mediated gene transfer transfectant panel, conventional somatic-cell hybrids containing well-defined breaks on chromosome 17, and in situ hybridization, we propose the following order for these loci: pter-(TP53-RNP2-D17S1)-(MYH2-MYH1)-D17Z 1-CRYB1-(ERBA1-GCSF-NGL)-acute promyelocytic leukemia breakpoint-RNU2-HOX2-(NGFR-COLIAI-MPO)-GAA-UM PH-GHC-TK1-GALK-qter. Using chromosome-mediated gene transfer, we have also regionally localized the random probes D17S6 to D17S19 on chromosome 17.

MeSH Terms
Animals Blotting, Southern Chromosome Mapping Chromosomes, Human, Pair 17 Gene Frequency Genetic Markers/analysis Humans Hybrid Cells/cytology Lymphocytes/cytology Nucleic Acid Hybridization Transfection
Chemicals
Genetic Markers
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Xu W M
Somatic Cell Genetics Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Gorman P A
Rider S H
Hedge P J
Moore G
Prichard C
Sheer D
Solomon E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.22.8563
Published
1988-11-00
Pages
8563-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282499
Subset
IM
Grants
Cancer Research UK · A3585 · United Kingdom
External Links
PubMed source DOI: 10.1073/pnas.85.22.8563 PMC Full Text View journal details
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