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PMID: 3148293 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Low- and intermediate-copy-number cloning vectors based on the Pseudomonas plasmid pVS1.

Antonie van Leeuwenhoek ·Vol. 54 ·No. 6 ·1988-00-00 ·Pages 567-73

Itoh Y, Soldati L, Leisinger T, Haas D

Abstract

The cloning vector pME290 (6.8 kb), which is derived from the Pseudomonas plasmid pVS1 and has about 7 copies, was mutagenized in vitro to provide derivatives with altered copy numbers. Thus, pME292 (about 1-3 copies) and pME294 (about 15-20 copies) were isolated. These vectors were used in the characterization of the P. aeruginosa argF gene encoding ornithine carbamoyltransferase.

MeSH Terms
Cloning, Molecular DNA, Bacterial/ultrastructure DNA, Recombinant/ultrastructure Gene Expression Regulation Genetic Vectors Plasmids Pseudomonas aeruginosa/genetics
Chemicals
DNA, Bacterial DNA, Recombinant
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Itoh Y
Dept. of Bacteriology, Shinshu University School of Medicine, Matsumoto, Japan.
Soldati L
Leisinger T
Haas D
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18 references, click to expand
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Article Info
Journal
Antonie van Leeuwenhoek
Abbr.
Antonie Van Leeuwenhoek
ISSN
0003-6072
DOI
10.1007/BF00588392
Published
1988-00-00
Pages
567-73
Language
English
Region
Netherlands
NLM ID
0372625
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00588392 View journal details
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