Home LiteratureArticle Details
PMID: 3146019 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The mglB sequence of Salmonella typhimurium LT2; promoter analysis by gene fusions and evidence for a divergently oriented gene coding for the mgl repressor.

Molecular & general genetics : MGG ·Vol. 214 ·No. 3 ·1988-11-00 ·Pages 579-87

Benner-Luger D, Boos W

Abstract

The mglB gene of Salmonella typhimurium LT2 coding for the galactose-binding protein (GBP) was sequenced. We compared the deduced amino acid sequence with the GBP sequence of Escherichia coli K 12. The mature proteins differ in only 19 of 309 amino acid residues, corresponding to 94% homology. Analysis of the mglB control region by promoter-probe vectors revealed that two promoters, P1 and P2, constitute the mgl control region (Pmgl). P1 and P2 function in a synergistic way. P1 is the main promoter of the operon; its activity is 20 times the activity of P2. Both promoters are activated by the cyclic adenosine monophosphate catabolite activator protein (cAMP/CAP) complex. While P1 is inactive in the absence of the cAMP/CAP complex, there is residual activity of P2 under these conditions. Studies on the inducibility of the mglBAEC operon using multicopy plasmid promoter-probe vectors were hampered by the titration of the mgl repressor resulting in a partially constitutive expression of the mgl operon. The results indicate that only P1 is responding to induction by D-fucose. A weak promoter, PD, within the P1 region but divergent to it was found. PD is neither stimulated by the cAMP/CAP complex nor by D-fucose. We cloned the gene located downstream to PD and found it to strongly repress the expression of the mgl operon. We termed this gene mglD. The presence of D-fucose abolished the repression caused by the plasmid-encoded mglD gene product.

MeSH Terms
Amino Acid Sequence Base Sequence Calcium-Binding Proteins Carrier Proteins/genetics Cloning, Molecular DNA, Bacterial Escherichia coli/genetics Galactose Genes Genes, Bacterial Molecular Sequence Data Monosaccharide Transport Proteins Operon Periplasmic Binding Proteins Plasmids Promoter Regions, Genetic Repressor Proteins/genetics Restriction Mapping Salmonella typhimurium/genetics Sequence Homology, Nucleic Acid Transcription Factors/genetics Transcription, Genetic beta-Galactosidase/genetics
Chemicals
Calcium-Binding Proteins Carrier Proteins DNA, Bacterial Monosaccharide Transport Proteins Periplasmic Binding Proteins Repressor Proteins Transcription Factors galactose-binding protein beta-Galactosidase Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Benner-Luger D
Department of Biology, University of Konstanz, Federal Republic of Germany.
Boos W
References (42)
42 references, click to expand
  1. The nucleotide sequences defining the signal peptides of the galactose-binding protein and the arabinose-binding protein.
    J Biol Chem. 1983 Sep 25;258(18):10853-5 PMID: 6885805
  2. Transport systems for galactose and galactosides in Escherichia coli. I. Genetic determination and regulation of the methyl-galactoside permease.
    J Mol Biol. 1966 Mar;16(1):42-50 PMID: 5331243
  3. The 3 A resolution structure of a D-galactose-binding protein for transport and chemotaxis in Escherichia coli.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):1792-6 PMID: 6340108
  4. Isolation and complementation of mutants in galactose taxis and transport.
    J Bacteriol. 1974 Feb;117(2):509-16 PMID: 4359647
  5. Properties of mutants in galactose taxis and transport.
    J Bacteriol. 1974 Feb;117(2):517-26 PMID: 4359648
  6. Properties of the galactose binding protein of Salmonella typhimurium and Escherichia coli.
    Biochemistry. 1977 Feb 8;16(3):381-6 PMID: 319823
  7. Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli.
    Nature. 1984 Sep 20-26;311(5983):232-5 PMID: 6090927
  8. The amino acid sequence of the D-galactose-binding protein from Escherichia coli B/r.
    J Biol Chem. 1981 May 10;256(9):4350-6 PMID: 7012152
  9. Regulation of the Escherichia coli methylgalactoside transport system by gene mglD.
    J Bacteriol. 1975 Jul;123(1):69-74 PMID: 1095564
  10. Lambda placMu: a transposable derivative of bacteriophage lambda for creating lacZ protein fusions in a single step.
    J Bacteriol. 1984 Jun;158(3):1084-93 PMID: 6327627
  11. Mapping of mglB, the structural gene of the galactose-binding protein of Escherichia coli.
    Mol Gen Genet. 1981;184(3):508-18 PMID: 6278261
  12. The galactose binding protein and its relationship to the beta-methylgalactoside permease from Escherichia coli.
    Eur J Biochem. 1969 Aug;10(1):66-73 PMID: 4981309
  13. Probing the structure of gal operator-repressor complexes. Conformation change in DNA.
    J Biol Chem. 1987 Sep 25;262(27):13258-62 PMID: 3308875
  14. The x-ray structure of the periplasmic galactose binding protein from Salmonella typhimurium at 3.0-A resolution.
    J Biol Chem. 1983 Jul 10;258(13):7991-7 PMID: 6345536
  15. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  16. DNA-protein recognition: demonstration of three genetically separated operator elements that are required for repression of the Escherichia coli deoCABD promoters by the DeoR repressor.
    EMBO J. 1986 Aug;5(8):2015-21 PMID: 3019678
  17. Phasmid vectors for identification of genes by complementation of Escherichia coli mutants.
    J Bacteriol. 1985 May;162(2):777-83 PMID: 2985547
  18. Cloning of mglB, the structural gene for the galactose-binding protein of Salmonella typhimurium and Escherichia coli.
    Mol Gen Genet. 1982;185(3):473-80 PMID: 6285145
  19. A novel calcium binding site in the galactose-binding protein of bacterial transport and chemotaxis.
    Nature. 1987 Jun 18-24;327(6123):635-8 PMID: 3600760
  20. Temperature-sensitive catabolite activator protein in Escherichia coli BUG6.
    J Bacteriol. 1985 Jan;161(1):347-52 PMID: 3155717
  21. Sequence of the mglB gene from Escherichia coli K12: comparison of wild-type and mutant galactose chemoreceptors.
    Mol Gen Genet. 1987 Jun;208(1-2):247-53 PMID: 3302609
  22. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  23. Characterization of the Salmonella typhimurium mgl operon and its gene products.
    J Bacteriol. 1985 Jul;163(1):37-45 PMID: 3924896
  24. A comparison of the amino-terminal sequences of several carbohydrate binding proteins from Escherichia coli and Salmonella typhimurium.
    FEBS Lett. 1977 Aug 15;80(2):377-9 PMID: 330247
  25. Restriction map of the Escherichia coli malA region and identification of the malT product.
    J Bacteriol. 1980 Aug;143(2):761-71 PMID: 6259122
  26. Galactose transport in Salmonella typhimurium.
    J Bacteriol. 1977 Feb;129(2):630-9 PMID: 190207
  27. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  28. Gene regulation by proteins acting nearby and at a distance.
    Nature. 1986 Aug 21-27;322(6081):697-701 PMID: 3018583
  29. Endogenous induction of the galactose operon in Escherichia coli K12.
    Proc Natl Acad Sci U S A. 1966 Mar;55(3):622-9 PMID: 5329012
  30. Transport of sugars and amino acids in bacteria. I. Purification and specificity of the galactose- and leucine-binding proteins.
    J Biol Chem. 1968 Jun 10;243(11):3116-22 PMID: 4871201
  31. Role of the galactose binding protein in chemotaxis of Escherichia coli toward galactose.
    Nat New Biol. 1971 Mar 24;230(12):101-4 PMID: 4927373
  32. The structure of D-galactose-binding protein at 4.1 A resolution looks like L-arabinose-binding protein.
    J Biol Chem. 1980 Jul 25;255(14):6559-51 PMID: 6993475
  33. Promoter-probe vectors for the analysis of divergently arranged promoters.
    Gene. 1986;42(1):37-48 PMID: 3087817
  34. Direction of transcription of the regulatory gene araC in Escherichia coli B-r.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3635-9 PMID: 4610582
  35. Cloning and promoter identification of the iron-regulated cir gene of Escherichia coli.
    J Bacteriol. 1987 Dec;169(12):5343-52 PMID: 3316180
  36. Identification of the mglA gene product in the beta-methylgalactoside transport system of Escherichia coli using plasmid DNA deletions generated in vitro.
    J Biol Chem. 1982 Aug 10;257(15):9030-4 PMID: 6807987
  37. Characterization of the mgl operon of Escherichia coli by transposon mutagenesis and molecular cloning.
    J Bacteriol. 1983 Jan;153(1):408-15 PMID: 6294056
  38. The regulation of the beta-methylgalactoside transport system and of the galactose binding protein of Escherichia coli K12.
    Eur J Biochem. 1971 Apr 30;19(4):457-70 PMID: 4996112
  39. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
    Gene. 1983 Dec;26(1):101-6 PMID: 6323249
  40. Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasmid copy number of pBR322 and its derivatives.
    Mol Gen Genet. 1986 Nov;205(2):285-90 PMID: 3100913
  41. Changes in the specific activities of the galactose enzymes in E. coli under different growth conditions.
    Mol Gen Genet. 1967;100(2):203-9 PMID: 4869991
  42. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
    J Mol Biol. 1976 Jul 5;104(3):541-55 PMID: 781293
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330498
Published
1988-11-00
Pages
579-87
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330498
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2