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PMID: 3137211 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Efficiency of homologous DNA recombination varies along the Bacillus subtilis chromosome.

Journal of bacteriology ·Vol. 170 ·No. 9 ·1988-09-00 ·Pages 3978-82

Vagner V, Ehrlich SD

Abstract

Structures consisting of a genetic marker (erythromycin or kanamycin resistance, thymidylate synthetase) flanked by 3.4-kilobase direct repeats (pBR322 sequences) were inserted in 12 different locations of the Bacillus subtilis chromosome. Recombination between the repeats was followed by the loss of the genetic marker. Recombination frequencies found in different locations varied from 1.2 X 10(-5) to 40 X 10(-5) per cell generation. Such differences were highly significant (P less than 0.001).

MeSH Terms
Bacillus subtilis/genetics Chromosomes, Bacterial DNA, Bacterial/genetics Drug Resistance, Microbial/genetics Genetic Markers Nucleic Acid Hybridization Plasmids Recombination, Genetic Repetitive Sequences, Nucleic Acid Thymidylate Synthase/genetics Transformation, Bacterial
Chemicals
DNA, Bacterial Genetic Markers Thymidylate Synthase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vagner V
Institut des Biotechnologies, Centre de Recherche de Jouy en Josas, France.
Ehrlich S D
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.9.3978-3982.1988
Published
1988-09-00
Pages
3978-82
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211398
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.170.9.3978-3982.1988 PMC Full Text View journal details
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