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PMID: 3129728 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human thymic epithelial cells directly induce activation of autologous immature thymocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 9 ·1988-05-00 ·Pages 3125-9

Denning SM, Kurtzberg J, Le PT, Tuck DT, Singer KH, Haynes BF

Abstract

To study the role that epithelial cells of the thymic microenvironment play in promoting activation of immature CD7+, CD2+, CD4-, CD8- (double-negative) human thymocytes, we have isolated thymocyte subsets from normal postnatal thymus and have cocultured autologous double-negative thymocytes with pure populations of thymic epithelial (TE) cells. We report that TE cells directly activate double-negative thymocytes to proliferate and that TE cells enhance the ability of double-negative thymocytes to proliferate in response to stimulation with exogenous interleukin 2. Activated double-negative thymocytes that proliferated in vitro in the presence of TE cells and interleukin 2 remained double-negative after 23 days in culture. Moreover, TE-cell culture supernatants in the absence of intact TE cells contain interleukin 1, interleukin 3, and granulocyte/macrophage-colony-stimulating factor activity for human bone marrow cells and can activate double-negative thymocytes to proliferate. Antibodies against interleukin 1 and against granulocyte/macrophage-colony-stimulating factor inhibited TE-cell-induced thymocyte activation. These data indicate that one role of TE cells in vivo may be to activate double-negative thymocytes to proliferate.

MeSH Terms
Antigens, Differentiation, T-Lymphocyte/analysis Cell Communication Cell Differentiation Cell Division Epithelial Cells Humans Interleukin-2/pharmacology Receptors, Immunologic/biosynthesis Receptors, Interleukin-2 T-Lymphocytes/cytology Thymidine/metabolism Thymus Gland/cytology
Chemicals
Antigens, Differentiation, T-Lymphocyte Interleukin-2 Receptors, Immunologic Receptors, Interleukin-2 Thymidine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Denning S M
Department of Medicine, Duke University School of Medicine, Durham, NC 27710.
Kurtzberg J
Le P T
Tuck D T
Singer K H
Haynes B F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.9.3125
Published
1988-05-00
Pages
3125-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280156
Subset
IM
Grants
NHLBI NIH HHS · 2T32 HL07101 · United States
NIADDK NIH HHS · AMR34808 · United States
NCI NIH HHS · CA28936 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.9.3125 PMC Full Text View journal details
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