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PMID: 3110780 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning of human thymic subcapsular cortex epithelial cells with T-lymphocyte binding sites and hemopoietic growth factor activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 14 ·1987-07-00 ·Pages 4999-5003

Mizutani S, Watt SM, Robertson D, Hussein S, Healy LE, Furley AJ, Greaves MF

Abstract

The thymic microenvironment involves complex cell interactions among different types of epithelial cells, macrophages, tissue histiocytes, and immature and maturing T cells. We describe the isolation of a subset of thymic epithelial cells by selective primary culture followed by cotransfection with a simian virus 40 replication-origin-defective mutant and pSV2neo plasmid. The cloned cells have the composite immunophenotype that is unique to thymic subcapsular epithelial cells, suggesting that they may provide a model system in vitro for analyzing the earliest steps in T-cell differentiation. This possibility is supported by the finding that these epithelial cells express LFA-3-associated binding sites for T cells, secrete a macrophage hemopoietic growth factor, and synergize with macrophages in the production of interleukin 1.

MeSH Terms
Antigens, Differentiation, T-Lymphocyte Antigens, Surface/analysis Cell Differentiation Clone Cells/cytology,metabolism Colony-Stimulating Factors/metabolism Epithelial Cells Epithelium/metabolism Hematopoietic Stem Cells/cytology,metabolism Humans Interleukin-1/biosynthesis Lymphocyte Function-Associated Antigen-1 Macrophages/metabolism T-Lymphocytes/cytology Thymus Gland/cytology
Chemicals
Antigens, Differentiation, T-Lymphocyte Antigens, Surface Colony-Stimulating Factors Interleukin-1 Lymphocyte Function-Associated Antigen-1
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Mizutani S
Watt S M
Robertson D
Hussein S
Healy L E
Furley A J
Greaves M F
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41 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.14.4999
Published
1987-07-00
Pages
4999-5003
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC305234
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.14.4999 PMC Full Text View journal details
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