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PMID: 3119564 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Cell-cycle-specific fluctuation in cytoplasmic membrane composition in aerobically grown Rhodospirillum rubrum.

Journal of bacteriology ·Vol. 169 ·No. 12 ·1987-12-00 ·Pages 5445-51

Myers CR, Collins ML

Abstract

Aerobic growth with synchronous cell division was induced in Rhodospirillum rubrum by starvation methods. Cells were harvested at different points in the cell cycle. Analysis of the composition of the cell envelope prepared by differential centrifugation or density gradient-purified cytoplasmic membrane obtained from cells at different times indicated that the protein/phospholipid ratio fluctuated with the cell cycle. The protein/phospholipid ratio of cell envelope from selection-synchronized cells also fluctuated with the cell cycle. These studies indicate that the phenomenon of cell-cycle-dependent fluctuation in membrane composition is not restricted to the intracytoplasmic chromatophore membrane of phototrophic cells.

MeSH Terms
Aerobiosis Bacterial Proteins/analysis Cell Cycle Cell Membrane/analysis Cell Wall/analysis Centrifugation, Density Gradient Membrane Lipids/analysis Membrane Proteins/analysis Phospholipids/analysis Rhodospirillum rubrum/analysis,cytology,ultrastructure
Chemicals
Bacterial Proteins Membrane Lipids Membrane Proteins Phospholipids
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Myers C R
Department of Biological Sciences, University of Wisconsin, Milwaukee 53201.
Collins M L
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.12.5445-5451.1987
Published
1987-12-00
Pages
5445-51
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213970
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.169.12.5445-5451.1987 PMC Full Text View journal details
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