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PMID: 3114742 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A second form of the beta subunit of signal-transducing G proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 17 ·1987-09-00 ·Pages 6122-5

Gao B, Gilman AG, Robishaw JD

Abstract

A complementary DNA (cDNA) has been isolated that encodes a second form of the beta subunit of signal-transducing guanine nucleotide-binding regulatory proteins (G proteins). The cDNA corresponds to a 1.8-kilobase mRNA, and nucleotide sequence analysis indicates that the encoded polypeptide consists of 340-amino acid residues with a Mr of 37,335. Although the deduced polypeptide is the same size as that reported previously for the beta subunit, 10% of the amino acid residues are different. Furthermore, the 5' and 3' non-translated regions of this cDNA show no significant homology with those reported previously for cDNAs that encode the beta subunit. These data refute prior conclusions that there is only one form of the G protein beta subunit.

MeSH Terms
Adrenal Glands/metabolism Amino Acid Sequence Animals Base Sequence Cattle Cloning, Molecular DNA/genetics GTP-Binding Proteins/genetics Humans Protein Conformation
Chemicals
DNA GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gao B
Gilman A G
Robishaw J D
References (29)
29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.17.6122
Published
1987-09-00
Pages
6122-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299020
Subset
IM
Grants
NIGMS NIH HHS · GM09731 · United States
NIGMS NIH HHS · GM34497 · United States
Databases
GENBANK
J02956, M16538, M16539
External Links
PubMed source DOI: 10.1073/pnas.84.17.6122 PMC Full Text View journal details
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