Secretion of human serum albumin from Bacillus subtilis.

Journal of bacteriology ·Vol. 169 ·No. 7 ·1987-07-00 ·Pages 2917-25

Saunders CW, Schmidt BJ, Mallonee RL, Guyer MS

Abstract

We have fused the structural gene (hsa) for human serum albumin (HSA) to the expression elements and signal sequence coding region of each of two genes from Bacillus amyloliquefaciens P, an alpha-amylase gene (amyBamP) and a neutral protease gene (nprBamP). Bacillus subtilis strains harboring either of these gene fusions synthesized a protein with the antigenic characteristics and size (68 kilodaltons) of HSA. Results from pulse-labeling studies indicated that the bacterially produced HSA was secreted from cells which had been converted to protoplasts. Results from similar studies with intact cells suggested that the signal sequence was removed from the hybrid protein, providing further evidence that B. subtilis can translocate this foreign protein across the cell membrane. Signal sequence removal was efficient when the level of HSA synthesis was low. However, in strains which synthesized HSA at a high level, signal sequence removal was less efficient.

MeSH Terms

Bacillus subtilis/physiology Biological Transport Cell Membrane/metabolism Protein Processing, Post-Translational Protein Sorting Signals/genetics Recombinant Fusion Proteins/metabolism Recombinant Proteins/metabolism Serum Albumin/biosynthesis,metabolism

Chemicals

Protein Sorting Signals Recombinant Fusion Proteins Recombinant Proteins Serum Albumin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Saunders C W

Schmidt B J

Mallonee R L

Guyer M S

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36 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.169.7.2917-2925.1987

Published

1987-07-00

Pages

2917-25

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC212327

Subset

External Links

PubMed source DOI: 10.1128/jb.169.7.2917-2925.1987 PMC Full Text View journal details

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Secretion of human serum albumin from Bacillus subtilis.

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