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PMID: 3109890 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and light regulation of expression of the phycocyanin operon of the cyanobacterium Anabaena.

The EMBO journal ·Vol. 6 ·No. 4 ·1987-04-00 ·Pages 871-84

Belknap WR, Haselkorn R

Abstract

The biliprotein phycocyanin (PC) is a major constituent of the light-harvesting apparatus of cyanobacteria and red algae. A DNA fragment encoding the beta and alpha subunits of PC was isolated from a genomic library of the cyanobacterium Anabaena 7120 DNA. The single-copy PC genes are part of a larger operon which consists of five open reading frames (ORFs) encoding, in order, the beta and alpha subunits of PC, two linker polypeptides associated with PC in phycobilisome rods, and a fifth ORF, which may encode a linker polypeptide involved in attachment of the phycobilisome rod to the core of the structure. The operon yields three major transcripts, the first of which (1.4 kb) encodes only the PC subunits. A second (3.6 kb) encodes all five ORFs, and appears to arise from partial read-through of a terminator following the PC subunit genes. The third transcript (1.4 kb) encodes the last two ORFs. The relative levels of the three transcripts in vivo are modulated by light intensity, but they are not altered by the removal of fixed nitrogen from the growth medium. The site of light regulation appears to be the terminator following the PC genes, rather than a promoter.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Cyanobacteria/genetics,radiation effects Gene Expression Regulation Genes/radiation effects Light Macromolecular Substances Nucleic Acid Hybridization Operon/radiation effects Phycobilisomes Phycocyanin/genetics Pigments, Biological/genetics Transcription, Genetic
Chemicals
Macromolecular Substances Phycobilisomes Pigments, Biological Phycocyanin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Belknap W R
Haselkorn R
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38 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-04-00
Pages
871-84
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553477
Subset
IM
Grants
NIGMS NIH HHS · GM 21823 · United States
NICHD NIH HHS · HDO7136 · United States
Databases
GENBANK
X05239
External Links
PubMed source PMC Full Text View journal details
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