T-helper function of parent leads to F1 chimeras. Presence of a separate T-cell subgroup able to stimulate allogeneic B cells but not syngeneic B cells.

The Journal of experimental medicine ·Vol. 149 ·No. 2 ·1979-02-01 ·Pages 387-97

Sprent J, von Boehmer H

Abstract

Parent leads to F1 chimeras were prepared by reconstituting sublethally irradiated H-2 heterozygous mice with marrow cells from one parental strain. Purified parental strain T cells prepared from unprimed chimeras were exposed to sheep erythrocytes in heavily irradiated mice of each of the two parental strains and recovered from thoracic duct lymph of the recipients at either day 1 or day 5 posttransfer. The lymphoborne cells were then tested for their capacity to collaborate in vivo with B cells of the two parental strains. From this approach it was concluded that parent leads to F1 chimera T cells contain two discrete subgroups of T-helper cells, one specific for self H-2 determinants and the other restricted to H-2 determinants of the opposite parental strain. The restrictions mapped to the K-end of the H-2 complex.

MeSH Terms

Animals B-Lymphocytes/immunology Chromosome Mapping H-2 Antigens Homozygote Hybridization, Genetic Immunologic Memory Lymphocyte Cooperation Major Histocompatibility Complex Mice Radiation Chimera T-Lymphocytes/immunology

Chemicals

H-2 Antigens

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sprent J

von Boehmer H

References (25)

25 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.149.2.387

Published

1979-02-01

Pages

387-97

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2184813

Subset

External Links

PubMed source DOI: 10.1084/jem.149.2.387 PMC Full Text View journal details

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T-helper function of parent leads to F1 chimeras. Presence of a separate T-cell subgroup able to stimulate allogeneic B cells but not syngeneic B cells.

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