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PMID: 31084542 Published · ppublish English Journal Article

Sulforaphane Decrease of SERTAD1 Expression Triggers G1/S Arrest in Breast Cancer Cells.

Journal of medicinal food ·Vol. 22 ·No. 5 ·2019-05-00 ·Pages 444-450

Cheng AC, Shen CJ, Hung CM, Hsu YC

Abstract

Studies have identified the potential of chemopreventive effects of sulforaphane (SFN); however, the underlying mechanisms of its effect on breast cancer require further elucidation. This study investigated the anticancer effects of SFN that specifically induces G1/S arrest in breast ductal carcinoma (ZR-75-1) cells. The proliferation of the cancer cells after treatment with SFN was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. DNA content and cell cycle status were analyzed through flow cytometry. Our results demonstrated the inhibition of growth in ZR-75-1 cells upon SFN exposure. In addition, SERTAD1 (SEI-1) caused the accumulation of SFN-treated G1/S-phase cells. The downregulation of SEI-1, cyclin D2, and histone deacetylase 3 suggested that in addition to the identified effects of SFN against breast cancer prevention, it may also exert antitumor activities in established breast cancer cells. In conclusion, SFN can inhibit growth of and induce cell cycle arrest in cancer cells, suggesting its potential role as an anticancer agent.

MeSH Terms
Antineoplastic Agents, Phytogenic/pharmacology Breast Neoplasms/drug therapy,genetics,metabolism,physiopathology Cell Line, Tumor Cell Proliferation/drug effects Cyclin D2/genetics,metabolism G1 Phase Cell Cycle Checkpoints/drug effects Histone Deacetylases/genetics,metabolism Humans Isothiocyanates/pharmacology Nuclear Proteins/genetics,metabolism Plant Extracts/pharmacology S Phase Cell Cycle Checkpoints/drug effects Sulfoxides Trans-Activators/genetics,metabolism Transcription Factors Vegetables/chemistry
Chemicals
Antineoplastic Agents, Phytogenic Cyclin D2 Isothiocyanates Nuclear Proteins Plant Extracts SERTAD1 protein, human Sulfoxides Trans-Activators Transcription Factors Histone Deacetylases histone deacetylase 3 sulforaphane
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cheng An-Chin
1 Department of Nutrition and Health Sciences; College of Health Sciences; Chang Jung Christian University, Tainan, Taiwan.
Shen Ching-Ju
2 Department of Gynecology and Obstetrics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
Hung Chao-Ming
3 Department of General Surgery, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan.
Hsu Yi-Chiang
4 Department of Medical Sciences Industry, College of Health Sciences; Chang Jung Christian University, Tainan, Taiwan.
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Article Info
Journal
Journal of medicinal food
Abbr.
J Med Food
ISSN
1557-7600
DOI
10.1089/jmf.2018.4195
Published
2019-05-00
Pages
444-450
Language
English
Region
United States
NLM ID
9812512
PMCID
PMC6534085
External Links
PubMed source DOI: 10.1089/jmf.2018.4195 PMC Full Text View journal details
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