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PMID: 3104527 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Endocytosis and recycling of the T3-T cell receptor complex. The role of T3 phosphorylation.

The Journal of experimental medicine ·Vol. 165 ·No. 4 ·1987-04-01 ·Pages 1141-59

Krangel MS

Abstract

An assay has been developed to assess the dynamics of cell surface glycoproteins, in which neuraminidase digestion of intact cells is used to determine the fate of cell surface molecules initially labelled via lactoperoxidase-catalyzed iodination. This approach has been used to demonstrate the constitutive endocytosis and recycling of the T3-T cell receptor (T CR) complex on the human T leukemic cell line HPB-MLT. Stable populations of both phosphorylated and nonphosphorylated forms of the T3 gamma peptide have been identified in these cells. Whereas the former are constitutively endocytosed, the latter appear to be excluded from this pathway. The results presented indicate that T3 gamma phosphorylation may control the endocytosis and recycling of the T3-TCR complex on this cell line.

MeSH Terms
Antigens, Differentiation, T-Lymphocyte Antigens, Surface/metabolism Cell Line Endocytosis Humans Leukemia/pathology Neuraminidase/metabolism Phorbol Esters/pharmacology Phosphorylation Receptors, Antigen, T-Cell/metabolism Receptors, Transferrin/metabolism T-Lymphocytes/drug effects,immunology,metabolism
Chemicals
Antigens, Differentiation, T-Lymphocyte Antigens, Surface Phorbol Esters Receptors, Antigen, T-Cell Receptors, Transferrin Neuraminidase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Krangel M S
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.165.4.1141
Published
1987-04-01
Pages
1141-59
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188576
Subset
IM
Grants
NIGMS NIH HHS · GM-38308 · United States
External Links
PubMed source DOI: 10.1084/jem.165.4.1141 PMC Full Text View journal details
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